Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay
Author(s)Lee, Jeong Hoon; Cosgrove, Benjamin D.; Lauffenburger, Douglas A.; Han, Jongyoon
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In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fold enhancement in sensitivity. In addition, we shorten the assay time down to less than 10 min, with the sample volume requirements of down to 5 cells. This device could be a generic and powerful tool for diagnostics and systems biology studies at the single-cell level, if properly optimized and integrated with the cell culture microdevices.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering
Journal of the American Chemical Society
American Chemical Society
Lee, Jeong Hoon et al. “Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay.” Journal of the American Chemical Society 131.30 (2009): 10340-10341.
Author's final manuscript