| dc.contributor.author | Lee, Jeong Hoon | |
| dc.contributor.author | Cosgrove, Benjamin D. | |
| dc.contributor.author | Lauffenburger, Douglas A. | |
| dc.contributor.author | Han, Jongyoon | |
| dc.date.accessioned | 2010-12-17T21:26:12Z | |
| dc.date.available | 2010-12-17T21:26:12Z | |
| dc.date.issued | 2009-07 | |
| dc.identifier.issn | 0002-7863 | |
| dc.identifier.issn | 1520-5126 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/60317 | |
| dc.description.abstract | In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fold enhancement in sensitivity. In addition, we shorten the assay time down to less than 10 min, with the sample volume requirements of down to 5 cells. This device could be a generic and powerful tool for diagnostics and systems biology studies at the single-cell level, if properly optimized and integrated with the cell culture microdevices. | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (R01-EB005743) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (R01-CA119402) | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (P50-GM68672) | en_US |
| dc.description.sponsorship | Institute for Collaborative Biotechnologies | en_US |
| dc.language.iso | en_US | |
| dc.publisher | American Chemical Society | en_US |
| dc.relation.isversionof | http://pubs.acs.org/doi/abs/10.1021/ja902594f | en_US |
| dc.rights | Attribution-Noncommercial-Share Alike 3.0 Unported | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
| dc.source | Prof. Lauffenburger | en_US |
| dc.title | Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Lee, Jeong Hoon et al. “Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay.” Journal of the American Chemical Society 131.30 (2009): 10340-10341. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
| dc.contributor.approver | Lauffenburger, Douglas A. | |
| dc.contributor.mitauthor | Lauffenburger, Douglas A. | |
| dc.contributor.mitauthor | Cosgrove, Benjamin D. | |
| dc.relation.journal | Journal of the American Chemical Society | en_US |
| dc.eprint.version | Author's final manuscript | |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Lee, Jeong Hoon; Cosgrove, Benjamin D.; Lauffenburger, Douglas A.; Han, Jongyoon | en |
| mit.license | OPEN_ACCESS_POLICY | en_US |
| mit.metadata.status | Complete | |
