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dc.contributor.authorLee, Jeong Hoon
dc.contributor.authorCosgrove, Benjamin D.
dc.contributor.authorLauffenburger, Douglas A.
dc.contributor.authorHan, Jongyoon
dc.date.accessioned2010-12-17T21:26:12Z
dc.date.available2010-12-17T21:26:12Z
dc.date.issued2009-07
dc.identifier.issn0002-7863
dc.identifier.issn1520-5126
dc.identifier.urihttp://hdl.handle.net/1721.1/60317
dc.description.abstractIn this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fold enhancement in sensitivity. In addition, we shorten the assay time down to less than 10 min, with the sample volume requirements of down to 5 cells. This device could be a generic and powerful tool for diagnostics and systems biology studies at the single-cell level, if properly optimized and integrated with the cell culture microdevices.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R01-EB005743)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R01-CA119402)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (P50-GM68672)en_US
dc.description.sponsorshipInstitute for Collaborative Biotechnologiesen_US
dc.language.isoen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionofhttp://pubs.acs.org/doi/abs/10.1021/ja902594fen_US
dc.rightsAttribution-Noncommercial-Share Alike 3.0 Unporteden_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourceProf. Lauffenburgeren_US
dc.titleMicrofluidic Concentration-Enhanced Cellular Kinase Activity Assayen_US
dc.typeArticleen_US
dc.identifier.citationLee, Jeong Hoon et al. “Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay.” Journal of the American Chemical Society 131.30 (2009): 10340-10341.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.approverLauffenburger, Douglas A.
dc.contributor.mitauthorLauffenburger, Douglas A.
dc.contributor.mitauthorCosgrove, Benjamin D.
dc.relation.journalJournal of the American Chemical Societyen_US
dc.eprint.versionAuthor's final manuscript
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsLee, Jeong Hoon; Cosgrove, Benjamin D.; Lauffenburger, Douglas A.; Han, Jongyoonen
mit.licenseOPEN_ACCESS_POLICYen_US


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