Pulse-modulated second harmonic imaging microscope quantitatively demonstrates marked increase of collagen in tumor after chemotherapy
Author(s)Raja, Anju M.; Xu, Shuoyu; Sun, Wanxin; Zhou, Jianbiao; Tai, Dean C. S.; Chen, Chien Shing; Rajapakse, Jagath; So, Peter T. C.; Yu, Hanry; ... Show more Show less
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Pulse-modulated second harmonic imaging microscopes (PM-SHIMs) exhibit improved signal-to-noise ratio (SNR) over conventional SHIMs on sensitive imaging and quantification of weak collagen signals inside tissues. We quantify the spatial distribution of sparse collagen inside a xenograft model of human acute myeloid leukemia (AML) tumor specimens treated with a new drug against receptor tyrosine kinase (ABT-869), and observe a significant increase in collagen area percentage, collagen fiber length, fiber width, and fiber number after chemotherapy. This finding reveals new insights into tumor responses to chemotherapy and suggests caution in developing new drugs and therapeutic regimens against cancers.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
Journal of Biomedical Optics
Society of Photo-optical Instrumentation Engineers
Raja, Anju M. et al. “Pulse-modulated Second Harmonic Imaging Microscope Quantitatively Demonstrates Marked Increase of Collagen in Tumor After Chemotherapy.” Journal of Biomedical Optics 15.5 (2010) : 056016. © 2010 SPIE.
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