Light scattering of human red blood cells during metabolic remodeling of membrane

Author(s)
Park, YongKeun; Dasari, Ramachandra Rao; Best-Popescu, Catherine A.; Popescu, Gabriel
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Abstract
We present the light scattering properties of individual human red blood cells (RBCs). We show that both the RBC static and dynamic scattering signals are altered by adenosine 5’-triphosphate (ATP)-driven membrane metabolic remodeling. To measure the light scattering signal from individual RBCs, we use diffraction phase microscopy together with a Fourier transform light scattering technique. RBC cytosolic ATPs are both chemically and metabolically depleted, and the corresponding scattering signals are compared with the light scattering signal of normal RBCs having physiologic levels of ATP.
Date issued
2011-01
URI
http://hdl.handle.net/1721.1/65103
Department
Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Spectroscopy Laboratory
Journal
Journal of Biomedical Optics
Publisher
Society of Photo-optical Instrumentation Engineers
Citation
Park, YongKeun et al. “Light Scattering of Human Red Blood Cells During Metabolic Remodeling of the Membrane.” Journal of Biomedical Optics 16.1 (2011) : 011013. © 2011 Society of Photo-Optical Instrumentation Engineers.
Version: Final published version
ISSN
1083-3668
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