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dc.contributor.authorDanino, Tal
dc.contributor.authorVolfson, Dmitri
dc.contributor.authorBhatia, Sangeeta N.
dc.contributor.authorTsimring, Lev
dc.contributor.authorHasty, Jeff
dc.date.accessioned2011-10-17T14:33:20Z
dc.date.available2011-10-17T14:33:20Z
dc.date.issued2011-05
dc.date.submitted2010-10
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1721.1/66267
dc.description.abstractCells organize in complex three-dimensional patterns by interacting with proteins along with the surrounding extracellular matrix. This organization provides the mechanical and chemical cues that ultimately influence a cell's differentiation and function. Here, we computationally investigate the pattern formation process of vascular mesenchymal cells arising from their interaction with Bone Morphogenic Protein-2 (BMP-2) and its inhibitor, Matrix Gla Protein (MGP). Using a first-principles approach, we derive a reaction-diffusion model based on the biochemical interactions of BMP-2, MGP and cells. Simulations of the model exhibit a wide variety of three-dimensional patterns not observed in a two-dimensional analysis. We demonstrate the emergence of three types of patterns: spheres, tubes, and sheets, and show that the patterns can be tuned by modifying parameters in the model such as the degradation rates of proteins and chemotactic coefficient of cells. Our model may be useful for improved engineering of three-dimensional tissue structures as well as for understanding three dimensional microenvironments in developmental processes.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (GM69811)en_US
dc.description.sponsorshipUnited States. Dept. of Energy (DOE CSGF fellowship)en_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0020182en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/2.5/en_US
dc.sourcePLoSen_US
dc.titleIn-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensionsen_US
dc.typeArticleen_US
dc.identifier.citationDanino, Tal et al. “In-Silico Patterning of Vascular Mesenchymal Cells in Three Dimensions.” Ed. Mukund Thattai. PLoS ONE 6 (2011): e20182.en_US
dc.contributor.departmentHarvard University--MIT Division of Health Sciences and Technologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.approverBhatia, Sangeeta N.
dc.contributor.mitauthorBhatia, Sangeeta N.
dc.relation.journalPLoS ONEen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsDanino, Tal; Volfson, Dmitri; Bhatia, Sangeeta N.; Tsimring, Lev; Hasty, Jeffen
dc.identifier.orcidhttps://orcid.org/0000-0002-1293-2097
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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