Label-free biomarker sensing in undiluted serum with suspended microchannel resonators
Author(s)Brault, Norman D.; Jiang, Shaoyi; Von Muhlen, Marcio Goldani; Knudsen, Scott Michael; Manalis, Scott R.
DownloadManalis-2010-Label-Free Biomarker Sensing in Undiluted Serum.pdf (771.5Kb)
Open Access Policy
Creative Commons Attribution-Noncommercial-Share Alike
MetadataShow full item record
Improved methods are needed for routine, inexpensive monitoring of biomarkers that could facilitate earlier detection and characterization of cancer. Suspended microchannel resonators (SMRs) are highly sensitive, batch-fabricated microcantilevers with embedded microchannels that can directly quantify adsorbed mass via changes in resonant frequency. As in other label-free detection methods, biomolecular measurements in complex media such as serum are challenging due to high background signals from nonspecific binding. In this report, we demonstrate that carboxybetaine-derived polymers developed to adsorb directly onto SMR SiO[subscript 2] surfaces act as ultralow fouling and functionalizable surface coatings. Coupled with a reference microcantilever, this approach enables detection of activated leukocyte cell adhesion molecule (ALCAM), a model cancer biomarker, in undiluted serum with a limit of detection of 10 ng/mL.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
American Chemical Society
von Muhlen, Marcio G. et al. “Label-Free Biomarker Sensing in Undiluted Serum with Suspended Microchannel Resonators.” Analytical Chemistry 82 (2010): 1905-1910. Web. 16 Nov. 2011. © 2010 American Chemical Society
Author's final manuscript