Label-free biomarker sensing in undiluted serum with suspended microchannel resonators
Author(s)Brault, Norman D.; Jiang, Shaoyi; Von Muhlen, Marcio Goldani; Knudsen, Scott Michael; Manalis, Scott R.
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Improved methods are needed for routine, inexpensive monitoring of biomarkers that could facilitate earlier detection and characterization of cancer. Suspended microchannel resonators (SMRs) are highly sensitive, batch-fabricated microcantilevers with embedded microchannels that can directly quantify adsorbed mass via changes in resonant frequency. As in other label-free detection methods, biomolecular measurements in complex media such as serum are challenging due to high background signals from nonspecific binding. In this report, we demonstrate that carboxybetaine-derived polymers developed to adsorb directly onto SMR SiO[subscript 2] surfaces act as ultralow fouling and functionalizable surface coatings. Coupled with a reference microcantilever, this approach enables detection of activated leukocyte cell adhesion molecule (ALCAM), a model cancer biomarker, in undiluted serum with a limit of detection of 10 ng/mL.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
American Chemical Society
von Muhlen, Marcio G. et al. “Label-Free Biomarker Sensing in Undiluted Serum with Suspended Microchannel Resonators.” Analytical Chemistry 82 (2010): 1905-1910. Web. 16 Nov. 2011. © 2010 American Chemical Society
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