A research-inspired laboratory sequence investigating acquired drug resistance
Author(s)Taylor, Elizabeth Vogel; Fortune, Jennifer A.; Drennan, Catherine L.
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Here, we present a six-session laboratory exercise designed to introduce students to standard biochemical techniques in the context of investigating a high impact research topic, acquired resistance to the cancer drug Gleevec. Students express a Gleevec-resistant mutant of the Abelson tyrosine kinase domain, the active domain of an oncogenic protein implicated in chronic myelogenous leukemia, and investigate the kinase activity of wild type and mutant enzyme in the presence of two cancer drugs. Techniques covered include protein expression, purification, and gel analysis, kinase activity assays, and protein structure viewing. The exercises provide students with a hands-on understanding of the impact of biochemistry on human health, and demonstrate their potential as the next generation of investigators.
DepartmentMassachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Biochemistry and Molecular Biology Education
John Wiley & Sons
Taylor, E. V., Fortune, J. A. and Drennan, C. L. (2010), A research-inspired laboratory sequence investigating acquired drug resistance. Biochemistry and Molecular Biology Education, 38: 247–252. doi: 10.1002/bmb.20384
Author's final manuscript