Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes

Author(s)
Goguen, Brenda N.; Hoffman, Brenton D.; Sellers, James R.; Schwartz, Martin A.; Imperiali, Barbara
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Abstract
Shining light on myosin: The incorporation of a caging group onto the essential phosphoserine residue of myosin by protein semisynthesis enables light-triggered activation of the protein (see picture). Caging eliminates the myosin activity, but exposure to 365 nm light restores its function to native levels. The caged protein can also be introduced into cells to facilitate studies of myosin with precise spatial and temporal resolution.
Date issued
2011-06
URI
http://hdl.handle.net/1721.1/69593
Department
Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Journal
Angewandte Chemie International Edition
Publisher
Wiley-VCH Verlag
Citation
Goguen, Brenda N. et al. “Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes.” Angewandte Chemie International Edition 50.25 (2011): 5667–5670.
Version: Author's final manuscript
ISSN
1433-7851
1521-3773
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