Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes
Author(s)Goguen, Brenda N.; Hoffman, Brenton D.; Sellers, James R.; Schwartz, Martin A.; Imperiali, Barbara
MetadataShow full item record
Shining light on myosin: The incorporation of a caging group onto the essential phosphoserine residue of myosin by protein semisynthesis enables light-triggered activation of the protein (see picture). Caging eliminates the myosin activity, but exposure to 365 nm light restores its function to native levels. The caged protein can also be introduced into cells to facilitate studies of myosin with precise spatial and temporal resolution.
DepartmentMassachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Angewandte Chemie International Edition
Goguen, Brenda N. et al. “Light-Triggered Myosin Activation for Probing Dynamic Cellular Processes.” Angewandte Chemie International Edition 50.25 (2011): 5667–5670.
Author's final manuscript