Anisotropic light scattering of individual sickle red blood cells
Author(s)Kim, Youngchan; Higgins, John M.; Dasari, Ramachandra Rao; Suresh, Subra; Park, YongKeun
MetadataShow full item record
We present the anisotropic light scattering of individual red blood cells (RBCs) from a patient with sickle cell disease (SCD). To measure light scattering spectra along two independent axes of elongated-shaped sickle RBCs with arbitrary orientation, we introduce the anisotropic Fourier transform light scattering (aFTLS) technique and measured both the static and dynamic anisotropic light scattering. We observed strong anisotropy in light scattering patterns of elongated-shaped sickle RBCs along its major axes using static aFTLS. Dynamic aFTLS analysis reveals the significantly altered biophysical properties in individual sickle RBCs. These results provide evidence that effective viscosity and elasticity of sickle RBCs are significantly different from those of the healthy RBCs.
DepartmentMassachusetts Institute of Technology. Department of Materials Science and Engineering; Massachusetts Institute of Technology. Spectroscopy Laboratory
Journal of Biomedical Optics
Kim, Youngchan et al. “Anisotropic Light Scattering of Individual Sickle Red Blood Cells.” Journal of Biomedical Optics 17.4 (2012): 040501. © 2012 Society of Photo-Optical Instrumentation Engineers
Final published version