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Base extrusion is found at helical junctions between right- and left-handed forms of DNA and RNA

Author(s)
Kim, Doyoun; Reddy, Sanjith; Kim, Kong Young; Rich, Alexander; Lee, Sangho; Kim, Kyeong Kyu; ... Show more Show less
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Abstract
Base extrusion is a major structural feature at the junction between B- and Z-DNA (the B–Z junction) where a base pair is broken, and the two bases are extruded from the double helix. Despite the demonstration of base extrusion at the B–Z junction, it is not clear whether a similar base extrusion occurs at other types of junctions involving the left-handed Z conformation. Here, we investigate structural changes of bases at three Z-form junctions: DNA B–Z and Z–Z and RNA A–Z junctions. By monitoring fluorescently labeled duplex nucleic acids using 2-aminopurines at various positions relative to the junction point, we show that base extrusion occurs not only at the DNA B–Z junction, but also at the RNA A–Z and DNA Z–Z junctions. Our data suggest that base extrusion is a general feature of Z-form nucleic-acid junctions.
Date issued
2009-05
URI
http://hdl.handle.net/1721.1/72438
Department
Massachusetts Institute of Technology. Department of Biology
Journal
Nucleic Acids Research
Publisher
Oxford University Press
Citation
Kim, D. et al. “Base Extrusion Is Found at Helical Junctions Between Right- and Left-handed Forms of DNA and RNA.” Nucleic Acids Research 37.13 (2009): 4353–4359. Web.
Version: Final published version
ISSN
0305-1048
1362-4962

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