A microfabricated deformability-based flow cytometer with application to malaria
Author(s)Bow, Hansen; Pivkin, Igor V.; Diez Silva, Monica; Goldfless, Stephen Jacob; Dao, Ming; Niles, Jacquin; Suresh, Subra; Han, Jongyoon; ... Show more Show less
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Malaria resulting from Plasmodium falciparum infection is a major cause of human suffering and mortality. Red blood cell (RBC) deformability plays a major role in the pathogenesis of malaria. Here we introduce an automated microfabricated “deformability cytometer” that measures dynamic mechanical responses of 10[superscript 3] to 10[superscript 4] individual RBCs in a cell population. Fluorescence measurements of each RBC are simultaneously acquired, resulting in a population-based correlation between biochemical properties, such as cell surface markers, and dynamic mechanical deformability. This device is especially applicable to heterogeneous cell populations. We demonstrate its ability to mechanically characterize a small number of P. falciparum-infected (ring stage) RBCs in a large population of uninfected RBCs. Furthermore, we are able to infer quantitative mechanical properties of individual RBCs from the observed dynamic behavior through a dissipative particle dynamics (DPD) model. These methods collectively provide a systematic approach to characterize the biomechanical properties of cells in a high-throughput manner.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Department of Materials Science and Engineering
Lab on a Chip
Royal Society of Chemistry
Bow, Hansen et al. “A Microfabricated Deformability-based Flow Cytometer with Application to Malaria.” Lab on a Chip 11.6 (2011): 1065.
Author's final manuscript