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Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator

dc.contributor.authorChen, Chia-Hung
dc.contributor.authorSarkar, Aniruddh
dc.contributor.authorSong, Yong-Ak
dc.contributor.authorMiller, Miles Aaron
dc.contributor.authorKim, Sung Jae
dc.contributor.authorGriffith, Linda G.
dc.contributor.authorLauffenburger, Douglas A.
dc.contributor.authorHan, Jongyoon
dc.date.accessioned2012-09-25T14:05:25Z
dc.date.available2012-09-25T14:05:25Z
dc.date.issued2011-06
dc.date.submitted2011-04
dc.identifier.issn0002-7863
dc.identifier.issn1520-5126
dc.identifier.urihttp://hdl.handle.net/1721.1/73163
dc.description.abstractWe introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to detect ultralow levels of matrix metalloproteinases (MMPs) from diluted cellular supernatant and showed that it significantly (~10-fold) reduced the time required to complete the assay and the sample volume used.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM68762)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant U54-CA112967)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01-EB010246)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01-GM081336)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Graduate Fellowship)en_US
dc.description.sponsorshipUnited States. Defense Advanced Research Projects Agency (Cipher Program)en_US
dc.language.isoen_US
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/ja2036628en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePubMed Centralen_US
dc.titleEnhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentratoren_US
dc.typeArticleen_US
dc.identifier.citationChen, Chia-Hung et al. “Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator.” Journal of the American Chemical Society 133.27 (2011): 10368–10371.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.mitauthorGoyal, Chia-Hung
dc.contributor.mitauthorSarkar, Aniruddh
dc.contributor.mitauthorSong, Yong-Ak
dc.contributor.mitauthorMiller, Miles Aaron
dc.contributor.mitauthorKim, Sung Jae
dc.contributor.mitauthorGriffith, Linda G.
dc.contributor.mitauthorLauffenburger, Douglas A.
dc.contributor.mitauthorHan, Jongyoon
dc.relation.journalJournal of the American Chemical Societyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsChen, Chia-Hung; Sarkar, Aniruddh; Song, Yong-Ak; Miller, Miles A.; Kim, Sung Jae; Griffith, Linda G.; Lauffenburger, Douglas A.; Han, Jongyoonen
dc.identifier.orcidhttps://orcid.org/0000-0002-1801-5548
dc.identifier.orcidhttps://orcid.org/0000-0001-7215-1439
dspace.mitauthor.errortrue
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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