Genetic engineering of human ES and iPS cells using TALE nucleases

Author(s)
Hockemeyer, Dirk; Wang, Haoyi; Kiani, Samira; Lai, Christine S.; Gao, Qing; ... Show more
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Abstract
Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
Date issued
2011-07
URI
http://hdl.handle.net/1721.1/73547
Department
Massachusetts Institute of Technology. Department of Biology; Whitehead Institute for Biomedical Research
Journal
Nature Biotechnology
Publisher
Nature Publishing Group
Citation
Hockemeyer, Dirk et al. “Genetic Engineering of Human Pluripotent Cells Using TALE Nucleases.” Nature Biotechnology 29.8 (2011): 731–734.
Version: Author's final manuscript
ISSN
1087-0156
1546-1696
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