Genetic engineering of human ES and iPS cells using TALE nucleases
Author(s)Hockemeyer, Dirk; Wang, Haoyi; Kiani, Samira; Lai, Christine S.; Gao, Qing; Cassady, John P.; Cost, Gregory J.; Zhang, Lei; Santiago, Yolanda; Miller, Jeffrey C.; Zeitler, Bryan; Cherone, Jennifer Michelle; Meng, Xiangdong; Hinkley, Sarah J.; Rebar, Edward J.; Gregory, Philip D.; Urnov, Fyodor D.; Jaenisch, Rudolf; ... Show more Show less
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Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
DepartmentMassachusetts Institute of Technology. Department of Biology; Whitehead Institute for Biomedical Research
Nature Publishing Group
Hockemeyer, Dirk et al. “Genetic Engineering of Human Pluripotent Cells Using TALE Nucleases.” Nature Biotechnology 29.8 (2011): 731–734.
Author's final manuscript