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Genetic engineering of human ES and iPS cells using TALE nucleases

dc.contributor.authorHockemeyer, Dirk
dc.contributor.authorWang, Haoyi
dc.contributor.authorKiani, Samira
dc.contributor.authorLai, Christine S.
dc.contributor.authorGao, Qing
dc.contributor.authorCassady, John P.
dc.contributor.authorCost, Gregory J.
dc.contributor.authorSantiago, Yolanda
dc.contributor.authorMiller, Jeffrey C.
dc.contributor.authorZeitler, Bryan
dc.contributor.authorCherone, Jennifer Michelle
dc.contributor.authorMeng, Xiangdong
dc.contributor.authorHinkley, Sarah J.
dc.contributor.authorRebar, Edward J.
dc.contributor.authorGregory, Philip D.
dc.contributor.authorUrnov, Fyodor D.
dc.contributor.authorJaenisch, Rudolf
dc.contributor.authorZhang, Lei, Ph. D Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, fl. 2014.
dc.date.accessioned2012-10-02T15:45:29Z
dc.date.available2012-10-02T15:45:29Z
dc.date.issued2011-07
dc.date.submitted2011-03
dc.identifier.issn1087-0156
dc.identifier.issn1546-1696
dc.identifier.urihttp://hdl.handle.net/1721.1/73547
dc.description.abstractTargeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R37-CA084198)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-CA087869)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant RO1-HD045022)en_US
dc.description.sponsorshipHoward Hughes Medical Instituteen_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nbt.1927en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alike 3.0en_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/3.0/en_US
dc.sourcePMCen_US
dc.titleGenetic engineering of human ES and iPS cells using TALE nucleasesen_US
dc.typeArticleen_US
dc.identifier.citationHockemeyer, Dirk et al. “Genetic Engineering of Human Pluripotent Cells Using TALE Nucleases.” Nature Biotechnology 29.8 (2011): 731–734.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorLai, Christine S.
dc.contributor.mitauthorCassady, John P.
dc.contributor.mitauthorJaenisch, Rudolf
dc.relation.journalNature Biotechnologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHockemeyer, Dirk; Wang, Haoyi; Kiani, Samira; Lai, Christine S; Gao, Qing; Cassady, John P; Cost, Gregory J; Zhang, Lei; Santiago, Yolanda; Miller, Jeffrey C; Zeitler, Bryan; Cherone, Jennifer M; Meng, Xiangdong; Hinkley, Sarah J; Rebar, Edward J; Gregory, Philip D; Urnov, Fyodor D; Jaenisch, Rudolfen
mit.licenseOPEN_ACCESS_POLICYen_US
mit.metadata.statusComplete


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