High-throughput in vivo vertebrate screening

Author(s)
Pardo-Martin, Carlos; Chang, Tsung-Yao; Koo, Bryan Kyo; Gilleland, Cody Lee; Wasserman, Steven Charles; ... Show more
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Abstract
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
Date issued
2010-07
URI
http://hdl.handle.net/1721.1/73602
Department
move to dc.description.sponsorship; Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Pardo-Martin, Carlos et al. “High-throughput in Vivo Vertebrate Screening.” Nature Methods 7.8 (2010): 634–636.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105
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