High-throughput in vivo vertebrate screening
Author(s)Pardo-Martin, Carlos; Chang, Tsung-Yao; Koo, Bryan Kyo; Gilleland, Cody Lee; Wasserman, Steven Charles; Yanik, Mehmet Fatih; ... Show more Show less
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We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
Departmentmove to dc.description.sponsorship; Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Nature Publishing Group
Pardo-Martin, Carlos et al. “High-throughput in Vivo Vertebrate Screening.” Nature Methods 7.8 (2010): 634–636.
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