| dc.contributor.author | Pardo-Martin, Carlos | |
| dc.contributor.author | Chang, Tsung-Yao | |
| dc.contributor.author | Koo, Bryan Kyo | |
| dc.contributor.author | Gilleland, Cody Lee | |
| dc.contributor.author | Wasserman, Steven Charles | |
| dc.contributor.author | Yanik, Mehmet Fatih | |
| dc.date.accessioned | 2012-10-04T16:15:36Z | |
| dc.date.available | 2012-10-04T16:15:36Z | |
| dc.date.issued | 2010-07 | |
| dc.date.submitted | 2010-01 | |
| dc.identifier.issn | 1548-7091 | |
| dc.identifier.issn | 1548-7105 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/73602 | |
| dc.description.abstract | We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery. | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (Director’s Innovator Award 1-DP2-OD002989–01) | en_US |
| dc.description.sponsorship | David & Lucile Packard Foundation (Award in Science and Engineering) | en_US |
| dc.description.sponsorship | Alfred P. Sloan Foundation (Award) | en_US |
| dc.description.sponsorship | Broad Institute of MIT and Harvard (Sparc Grant) | en_US |
| dc.description.sponsorship | National Science Foundation (U.S.) (Fellowship) | en_US |
| dc.description.sponsorship | Foxconn (Sponsorship) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Nature Publishing Group | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1038/nmeth.1481 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike 3.0 | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
| dc.source | PubMed Central | en_US |
| dc.title | High-throughput in vivo vertebrate screening | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Pardo-Martin, Carlos et al. “High-throughput in Vivo Vertebrate Screening.” Nature Methods 7.8 (2010): 634–636. | en_US |
| dc.contributor.department | Harvard University--MIT Division of Health Sciences and Technology | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biological Engineering | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
| dc.contributor.mitauthor | Pardo-Martin, Carlos | |
| dc.contributor.mitauthor | Chang, Tsung-Yao | |
| dc.contributor.mitauthor | Koo, Bryan Kyo | |
| dc.contributor.mitauthor | Gilleland, Cody Lee | |
| dc.contributor.mitauthor | Wasserman, Steven Charles | |
| dc.contributor.mitauthor | Yanik, Mehmet Fatih | |
| dc.relation.journal | Nature Methods | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Pardo-Martin, Carlos; Chang, Tsung-Yao; Koo, Bryan Kyo; Gilleland, Cody L; Wasserman, Steven C; Yanik, Mehmet Fatih | en |
| dc.identifier.orcid | https://orcid.org/0000-0002-5866-4606 | |
| dc.identifier.orcid | https://orcid.org/0000-0002-4612-1962 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
| mit.metadata.status | Complete | |
