Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters

Author(s)
Lukovic, Elvedin; Taylor, Elizabeth Vogel; Imperiali, Barbara
Thumbnail
DownloadImperiali_Monitoring protein.pdf (1.687Mb)
OPEN_ACCESS_POLICY
Terms of use
Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/
Metadata
Show full item record
Abstract
The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification.
Description
2010 September 13
Date issued
2009-08
URI
http://hdl.handle.net/1721.1/73713
Department
Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Journal
Angewandte Chemie International Edition
Publisher
Wiley Blackwell (John Wiley & Sons)
Citation
Luković, Elvedin, Elizabeth Vogel Taylor, and Barbara Imperiali. “Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters.” Angewandte Chemie International Edition 48.37 (2009): 6828–6831.
Version: Author's final manuscript
ISSN
1433-7851
1521-3773
Collections