Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters
Author(s)Lukovic, Elvedin; Taylor, Elizabeth Vogel; Imperiali, Barbara
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The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification.
2010 September 13
DepartmentMassachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Chemistry
Angewandte Chemie International Edition
Wiley Blackwell (John Wiley & Sons)
Luković, Elvedin, Elizabeth Vogel Taylor, and Barbara Imperiali. “Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters.” Angewandte Chemie International Edition 48.37 (2009): 6828–6831.
Author's final manuscript