| dc.contributor.author | Lukovic, Elvedin | |
| dc.contributor.author | Taylor, Elizabeth Vogel | |
| dc.contributor.author | Imperiali, Barbara | |
| dc.date.accessioned | 2012-10-10T14:57:05Z | |
| dc.date.available | 2012-10-10T14:57:05Z | |
| dc.date.issued | 2009-08 | |
| dc.date.submitted | 2009-06 | |
| dc.identifier.issn | 1433-7851 | |
| dc.identifier.issn | 1521-3773 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/73713 | |
| dc.description | 2010 September 13 | en_US |
| dc.description.abstract | The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification. | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.). Cell Migration Consortium (GM064346) | en_US |
| dc.description.sponsorship | National Science Foundation (U.S.). Biophysical Instrumentation Facility for the Study of Complex Macromolecular Systems (NSF-0070319) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | Wiley Blackwell (John Wiley & Sons) | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1002/anie.200902374 | en_US |
| dc.rights | Creative Commons Attribution-Noncommercial-Share Alike 3.0 | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-sa/3.0/ | en_US |
| dc.source | PMC | en_US |
| dc.title | Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Luković, Elvedin, Elizabeth Vogel Taylor, and Barbara Imperiali. “Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters.” Angewandte Chemie International Edition 48.37 (2009): 6828–6831. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Biology | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Chemistry | en_US |
| dc.contributor.mitauthor | Lukovic, Elvedin | |
| dc.contributor.mitauthor | Taylor, Elizabeth Vogel | |
| dc.contributor.mitauthor | Imperiali, Barbara | |
| dc.relation.journal | Angewandte Chemie International Edition | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Luković, Elvedin; Vogel Taylor, Elizabeth; Imperiali, Barbara | en |
| dc.identifier.orcid | https://orcid.org/0000-0002-5749-7869 | |
| dc.identifier.orcid | https://orcid.org/0000-0002-8121-9519 | |
| mit.license | OPEN_ACCESS_POLICY | en_US |
| mit.metadata.status | Complete | |
