Efficient replication bypass of size-expanded DNA base pairs in bacterial cells

Author(s)
Delaney, James C.; Gao, Jianmin; Liu, Haibo; Shrivastav, Nidhi; Essigmann, John M.; ... Show more
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Abstract
Supersize me! Size-expanded DNA bases (xDNA) are able to encode natural DNA sequences in replication. In vitro experiments with a DNA polymerase show nucleotide incorporation opposite the xDNA bases with correct pairing. In vivo experiments using E. coli show that two xDNA bases (xA and xC, see picture) encode the correct replication partners.
Description
Author Manuscript 2012 September 06
Date issued
2009-05
URI
http://hdl.handle.net/1721.1/75427
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemistry
Journal
Angewandte Chemie
Publisher
Wiley Blackwell
Citation
Delaney, James C. et al. “Efficient Replication Bypass of Size-Expanded DNA Base Pairs in Bacterial Cells.” Angewandte Chemie International Edition 48.25 (2009): 4524–4527.
Version: Author's final manuscript
ISSN
0044-8249
1521-3757
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