MIT Libraries logoDSpace@MIT

MIT
View Item 
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
  • DSpace@MIT Home
  • MIT Open Access Articles
  • MIT Open Access Articles
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Cell-surface sensors for real-time probing of cellular environments

Author(s)
Zhao, Weian; Schafer, Sebastian; Choi, Jonghoon; Yamanaka, Yvonne Joy; Lombardi, Maria L.; Bose, Suman; Carlson, Alicia L.; Phillips, Joseph A.; Teo, Weisuong; Droujinine, Ilia A.; Cui, Cheryl; Jain, Rakesh K.; Lammerding, Jan; Love, J. Christopher; Lin, Charles; Sarkar, Debanjan; Karnik, Rohit; Karp, Jeffrey Michael; ... Show more Show less
Thumbnail
DownloadKarnik_Cell-surface.pdf (2.152Mb)
OPEN_ACCESS_POLICY

Open Access Policy

Creative Commons Attribution-Noncommercial-Share Alike

Terms of use
Creative Commons Attribution-Noncommercial-Share Alike 3.0 http://creativecommons.org/licenses/by-nc-sa/3.0/
Metadata
Show full item record
Abstract
The ability to explore cell signalling and cell-to-cell communication is essential for understanding cell biology and developing effective therapeutics. However, it is not yet possible to monitor the interaction of cells with their environments in real time. Here, we show that a fluorescent sensor attached to a cell membrane can detect signalling molecules in the cellular environment. The sensor is an aptamer (a short length of single-stranded DNA) that binds to platelet-derived growth factor (PDGF) and contains a pair of fluorescent dyes. When bound to PDGF, the aptamer changes conformation and the dyes come closer to each other, producing a signal. The sensor, which is covalently attached to the membranes of mesenchymal stem cells, can quantitatively detect with high spatial and temporal resolution PDGF that is added in cell culture medium or secreted by neighbouring cells. The engineered stem cells retain their ability to find their way to the bone marrow and can be monitored in vivo at the single-cell level using intravital microscopy.
Description
Author Manuscript 2012 August 1.
Date issued
2011-07
URI
http://hdl.handle.net/1721.1/78868
Department
Harvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
Journal
Nature Nanotechnology
Publisher
Nature Publishing Group
Citation
Zhao, Weian, Sebastian Schafer, Jonghoon Choi, et al. 2011Cell-surface Sensors for Real-time Probing of Cellular Environments. Nature Nanotechnology 6(8): 524–531.
Version: Author's final manuscript
ISSN
1748-3387
1748-3395

Collections
  • MIT Open Access Articles

Browse

All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

Login

Statistics

OA StatisticsStatistics by CountryStatistics by Department
MIT Libraries
PrivacyPermissionsAccessibilityContact us
MIT
Content created by the MIT Libraries, CC BY-NC unless otherwise noted. Notify us about copyright concerns.