Cell-surface sensors for real-time probing of cellular environments
Author(s)Zhao, Weian; Schafer, Sebastian; Choi, Jonghoon; Yamanaka, Yvonne Joy; Lombardi, Maria L.; Bose, Suman; Carlson, Alicia L.; Phillips, Joseph A.; Teo, Weisuong; Droujinine, Ilia A.; Cui, Cheryl; Jain, Rakesh K.; Lammerding, Jan; Love, J. Christopher; Lin, Charles; Sarkar, Debanjan; Karnik, Rohit; Karp, Jeffrey Michael; ... Show more Show less
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The ability to explore cell signalling and cell-to-cell communication is essential for understanding cell biology and developing effective therapeutics. However, it is not yet possible to monitor the interaction of cells with their environments in real time. Here, we show that a fluorescent sensor attached to a cell membrane can detect signalling molecules in the cellular environment. The sensor is an aptamer (a short length of single-stranded DNA) that binds to platelet-derived growth factor (PDGF) and contains a pair of fluorescent dyes. When bound to PDGF, the aptamer changes conformation and the dyes come closer to each other, producing a signal. The sensor, which is covalently attached to the membranes of mesenchymal stem cells, can quantitatively detect with high spatial and temporal resolution PDGF that is added in cell culture medium or secreted by neighbouring cells. The engineered stem cells retain their ability to find their way to the bone marrow and can be monitored in vivo at the single-cell level using intravital microscopy.
Author Manuscript 2012 August 1.
DepartmentHarvard University--MIT Division of Health Sciences and Technology; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering
Nature Publishing Group
Zhao, Weian, Sebastian Schafer, Jonghoon Choi, et al. 2011Cell-surface Sensors for Real-time Probing of Cellular Environments. Nature Nanotechnology 6(8): 524–531.
Author's final manuscript