Automated whole-cell patch-clamp electrophysiology of neurons in vivo

Author(s)
Kodandaramaiah, Suhasa Bangalo; Talei Franzesi, Giovanni; Forest, Craig R.; Chow, Brian Yichiun; Boyden, Edward
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Abstract
Whole-cell patch-clamp electrophysiology of neurons is a gold-standard technique for high-fidelity analysis of the biophysical mechanisms of neural computation and pathology, but it requires great skill to perform. We have developed a robot that automatically performs patch clamping in vivo, algorithmically detecting cells by analyzing the temporal sequence of electrode impedance changes. We demonstrate good yield, throughput and quality of automated intracellular recording in mouse cortex and hippocampus.
Date issued
2012-05
URI
http://hdl.handle.net/1721.1/79604
Department
Massachusetts Institute of Technology. Media Laboratory; McGovern Institute for Brain Research at MIT; Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Kodandaramaiah, Suhasa B, Giovanni Talei Franzesi, Brian Y Chow, Edward S Boyden, and Craig R Forest. Automated Whole-cell Patch-clamp Electrophysiology of Neurons in Vivo. Nature Methods 9, no. 6 (May 6, 2012): 585-587.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105
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