AFB1-induced mutagenesis of the gpt gene in AS52 cells

Author(s)
Wattanawaraporn, Roongtiwa; Kim, Min Young; Adams, Jillian; Trudel, Laura J.; Woo, Leslie; ... Show more
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Abstract
Aflatoxin B1 (AFB1) is a potent mutagen and an important risk factor for hepatocellular carcinoma (HCC) in humans. Transgenic mouse strains and cells in culture have been used to detect different types of mutations caused by AFB1 and investigate the molecular determinants of their location and frequency. The AFB1 mutational spectrum in the gpt gene was markedly different in AS52 cells compared with the liver in gpt delta B6C3F1 transgenic mice. The results demonstrate the importance of metabolism, chromosomal location, transcription and selection conditions on mutational spectra.
Date issued
2012-08
URI
http://hdl.handle.net/1721.1/82021
Department
Massachusetts Institute of Technology. Center for Environmental Health Sciences; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemistry
Journal
Environmental and Molecular Mutagenesis
Publisher
John Wiley & Sons, Inc.
Citation
Wattanawaraporn, Roongtiwa, Min Young Kim, Jillian Adams, Laura J. Trudel, Leslie L. Woo, Robert G. Croy, John M. Essigmann, and Gerald N. Wogan. “AFB1-induced mutagenesis of the gpt gene in AS52 cells.” Environmental and Molecular Mutagenesis 53, no. 7 (August 26, 2012): 567-573.
Version: Author's final manuscript
ISSN
08936692
1098-2280
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