Using buoyant mass to measure the growth of single cells

Author(s)
Godin, Michel; Son, Sungmin; Tzur, Amit; Jorgensen, Paul; Kirschner, Marc W.; ... Show more
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Abstract
We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
Date issued
2010-04
URI
http://hdl.handle.net/1721.1/83911
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Microsystems Technology Laboratories; Koch Institute for Integrative Cancer Research at MIT
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Godin, Michel, Francisco Feijó Delgado, Sungmin Son, William H Grover, Andrea K Bryan, Amit Tzur, Paul Jorgensen, et al. “Using buoyant mass to measure the growth of single cells.” Nature Methods 7, no. 5 (April 11, 2010): 387-390.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105
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