Using buoyant mass to measure the growth of single cells
Author(s)Godin, Michel; Son, Sungmin; Tzur, Amit; Jorgensen, Paul; Kirschner, Marc W.; Delgado, Francisco Feijo; Grover, William H.; Bryan, Andrea Kristine; Payer, Kristofor Robert; Grossman, Alan D.; Manalis, Scott R.; ... Show more Show less
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We used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Biology; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Microsystems Technology Laboratories
Nature Publishing Group
Godin, Michel, Francisco Feijó Delgado, Sungmin Son, William H Grover, Andrea K Bryan, Amit Tzur, Paul Jorgensen, et al. “Using buoyant mass to measure the growth of single cells.” Nature Methods 7, no. 5 (April 11, 2010): 387-390.
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