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Using buoyant mass to measure the growth of single cells

dc.contributor.authorGodin, Michel
dc.contributor.authorSon, Sungmin
dc.contributor.authorTzur, Amit
dc.contributor.authorJorgensen, Paul
dc.contributor.authorKirschner, Marc W.
dc.contributor.authorDelgado, Francisco Feijo
dc.contributor.authorGrover, William H.
dc.contributor.authorBryan, Andrea Kristine
dc.contributor.authorPayer, Kristofor Robert
dc.contributor.authorGrossman, Alan D.
dc.contributor.authorManalis, Scott R.
dc.date.accessioned2014-01-13T19:06:33Z
dc.date.available2014-01-13T19:06:33Z
dc.date.issued2010-04
dc.date.submitted2009-12
dc.identifier.issn1548-7091
dc.identifier.issn1548-7105
dc.identifier.urihttp://hdl.handle.net/1721.1/83911
dc.description.abstractWe used a suspended microchannel resonator (SMR) combined with picoliter-scale microfluidic control to measure buoyant mass and determine the 'instantaneous' growth rates of individual cells. The SMR measures mass with femtogram precision, allowing rapid determination of the growth rate in a fraction of a complete cell cycle. We found that for individual cells of Bacillus subtilis, Escherichia coli, Saccharomyces cerevisiae and mouse lymphoblasts, heavier cells grew faster than lighter cells.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (MIT Center for Cell Decision Processes Grant P50GM68762)en_US
dc.description.sponsorshipUnited States. Army Research Office (Institute for Collaborative Biotechnologies Grant DAAD1903D0004)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nmeth.1452en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleUsing buoyant mass to measure the growth of single cellsen_US
dc.typeArticleen_US
dc.identifier.citationGodin, Michel, Francisco Feijó Delgado, Sungmin Son, William H Grover, Andrea K Bryan, Amit Tzur, Paul Jorgensen, et al. “Using buoyant mass to measure the growth of single cells.” Nature Methods 7, no. 5 (April 11, 2010): 387-390.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Microsystems Technology Laboratoriesen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorGodin, Michelen_US
dc.contributor.mitauthorDelgado, Francisco Feijoen_US
dc.contributor.mitauthorSon, Sungminen_US
dc.contributor.mitauthorGrover, William H.en_US
dc.contributor.mitauthorBryan, Andrea Kristineen_US
dc.contributor.mitauthorPayer, Kristofor Roberten_US
dc.contributor.mitauthorGrossman, Alan D.en_US
dc.contributor.mitauthorManalis, Scott R.en_US
dc.relation.journalNature Methodsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsGodin, Michel; Delgado, Francisco Feijó; Son, Sungmin; Grover, William H; Bryan, Andrea K; Tzur, Amit; Jorgensen, Paul; Payer, Kris; Grossman, Alan D; Kirschner, Marc W; Manalis, Scott Ren_US
dc.identifier.orcidhttps://orcid.org/0000-0001-5223-9433
dc.identifier.orcidhttps://orcid.org/0000-0002-8235-7227
dspace.mitauthor.errortrue
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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