High-resolution whole organ imaging using two-photon tissue cytometry
Author(s)
Ragan, Timothy; Sylvan, Jeremy D.; Kim, Ki Hean; Huang, Hayden; Bahlmann, Karsten; Lee, Richard T.; So, Peter T. C.; ... Show more Show less
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Show full item recordAbstract
Three-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer’s disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.
Date issued
2007-02Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Mechanical EngineeringJournal
Journal of Biomedical Optics
Publisher
SPIE
Citation
Ragan, Timothy, Jeremy D. Sylvan, Ki Hean Kim, Hayden Huang, Karsten Bahlmann, Richard T. Lee, and Peter T. C. So. “High-Resolution Whole Organ Imaging Using Two-Photon Tissue Cytometry.” Journal of Biomedical Optics 12, no. 1 (2007): 014015. © 2007 Society of Photo-Optical Instrumentation Engineers
Version: Final published version
ISSN
10833668
1560-2281