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dc.contributor.authorRagan, Timothy
dc.contributor.authorSylvan, Jeremy D.
dc.contributor.authorKim, Ki Hean
dc.contributor.authorHuang, Hayden
dc.contributor.authorBahlmann, Karsten
dc.contributor.authorLee, Richard T.
dc.contributor.authorSo, Peter T. C.
dc.date.accessioned2014-06-05T15:46:25Z
dc.date.available2014-06-05T15:46:25Z
dc.date.issued2007-02
dc.date.submitted2006-10
dc.identifier.issn10833668
dc.identifier.issn1560-2281
dc.identifier.urihttp://hdl.handle.net/1721.1/87650
dc.description.abstractThree-dimensional (3-D) tissue imaging offers substantial benefits to a wide range of biomedical investigations from cardiovascular biology, diabetes, Alzheimer’s disease to cancer. Two-photon tissue cytometry is a novel technique based on high-speed multiphoton microscopy coupled with automated histological sectioning, which can quantify tissue morphology and physiology throughout entire organs with subcellular resolution. Furthermore, two-photon tissue cytometry offers all the benefits of fluorescence-based approaches including high specificity and sensitivity and appropriateness for molecular imaging of gene and protein expression. We use two-photon tissue cytometry to image an entire mouse heart at subcellular resolution to quantify the 3-D morphology of cardiac microvasculature and myocyte morphology spanning almost five orders of magnitude in length scales.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R43 CA097670-01)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (R44 CA097670-02)en_US
dc.language.isoen_US
dc.publisherSPIEen_US
dc.relation.isversionofhttp://dx.doi.org/10.1117/1.2435626en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceSPIEen_US
dc.titleHigh-resolution whole organ imaging using two-photon tissue cytometryen_US
dc.typeArticleen_US
dc.identifier.citationRagan, Timothy, Jeremy D. Sylvan, Ki Hean Kim, Hayden Huang, Karsten Bahlmann, Richard T. Lee, and Peter T. C. So. “High-Resolution Whole Organ Imaging Using Two-Photon Tissue Cytometry.” Journal of Biomedical Optics 12, no. 1 (2007): 014015. © 2007 Society of Photo-Optical Instrumentation Engineersen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Mechanical Engineeringen_US
dc.contributor.mitauthorRagan, Timothyen_US
dc.contributor.mitauthorBahlmann, Karstenen_US
dc.contributor.mitauthorSo, Peter T. C.en_US
dc.relation.journalJournal of Biomedical Opticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsRagan, Timothy; Sylvan, Jeremy D.; Kim, Ki Hean; Huang, Hayden; Bahlmann, Karsten; Lee, Richard T.; So, Peter T. C.en_US
dc.identifier.orcidhttps://orcid.org/0000-0003-4698-6488
mit.licensePUBLISHER_POLICYen_US


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