Microfluidics-Based Assessment of Cell Deformability
Author(s)Adamo, Andrea; Sharei, Armon Reza; Adamo, Luigi; Lee, ByungKun; Mao, Shirley; Jensen, Klavs F.; ... Show more Show less
MetadataShow full item record
Mechanical properties of cells have been shown to have a significant role in disease, as in many instances cell stiffness changes when a cell is no longer healthy. We present a high-throughput microfluidics-based approach that exploits the connection between travel time of a cell through a narrow passage and cell stiffness. The system resolves both cell travel time and relative cell diameter while retaining information on the cell level. We show that stiffer cells have longer transit times than less stiff ones and that cell size significantly influences travel times. Experiments with untreated HeLa cells and cells made compliant with latrunculin A and cytochalasin B further demonstrate that travel time is influenced by cell stiffness, with the compliant cells having faster transit time.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
American Chemical Society (ACS)
Adamo, Andrea, Armon Sharei, Luigi Adamo, ByungKun Lee, Shirley Mao, and Klavs F. Jensen. “Microfluidics-Based Assessment of Cell Deformability.” Analytical Chemistry 84, no. 15 (August 7, 2012): 6438–6443. © 2012 American Chemical Society.
Author's final manuscript