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dc.contributor.authorAdamo, Andrea
dc.contributor.authorSharei, Armon Reza
dc.contributor.authorAdamo, Luigi
dc.contributor.authorLee, ByungKun
dc.contributor.authorMao, Shirley
dc.contributor.authorJensen, Klavs F
dc.date.accessioned2014-11-07T15:18:39Z
dc.date.available2014-11-07T15:18:39Z
dc.date.issued2012-08
dc.date.submitted2012-01
dc.identifier.issn0003-2700
dc.identifier.issn1520-6882
dc.identifier.urihttp://hdl.handle.net/1721.1/91491
dc.description.abstractMechanical properties of cells have been shown to have a significant role in disease, as in many instances cell stiffness changes when a cell is no longer healthy. We present a high-throughput microfluidics-based approach that exploits the connection between travel time of a cell through a narrow passage and cell stiffness. The system resolves both cell travel time and relative cell diameter while retaining information on the cell level. We show that stiffer cells have longer transit times than less stiff ones and that cell size significantly influences travel times. Experiments with untreated HeLa cells and cells made compliant with latrunculin A and cytochalasin B further demonstrate that travel time is influenced by cell stiffness, with the compliant cells having faster transit time.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH grant RC1 EB011187-02)en_US
dc.language.isoen_US
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/ac300264ven_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleMicrofluidics-Based Assessment of Cell Deformabilityen_US
dc.typeArticleen_US
dc.identifier.citationAdamo, Andrea, Armon Sharei, Luigi Adamo, ByungKun Lee, Shirley Mao, and Klavs F. Jensen. “Microfluidics-Based Assessment of Cell Deformability.” Analytical Chemistry 84, no. 15 (August 7, 2012): 6438–6443. © 2012 American Chemical Society.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorAdamo, Andreaen_US
dc.contributor.mitauthorSharei, Armon Rezaen_US
dc.contributor.mitauthorLee, ByungKunen_US
dc.contributor.mitauthorMao, Shirleyen_US
dc.contributor.mitauthorJensen, Klavs F.en_US
dc.relation.journalAnalytical Chemistryen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsAdamo, Andrea; Sharei, Armon; Adamo, Luigi; Lee, ByungKun; Mao, Shirley; Jensen, Klavs F.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7192-580X
dc.identifier.orcidhttps://orcid.org/0000-0001-5262-2264
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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