dc.contributor.author | Adamo, Andrea | |
dc.contributor.author | Sharei, Armon Reza | |
dc.contributor.author | Adamo, Luigi | |
dc.contributor.author | Lee, ByungKun | |
dc.contributor.author | Mao, Shirley | |
dc.contributor.author | Jensen, Klavs F | |
dc.date.accessioned | 2014-11-07T15:18:39Z | |
dc.date.available | 2014-11-07T15:18:39Z | |
dc.date.issued | 2012-08 | |
dc.date.submitted | 2012-01 | |
dc.identifier.issn | 0003-2700 | |
dc.identifier.issn | 1520-6882 | |
dc.identifier.uri | http://hdl.handle.net/1721.1/91491 | |
dc.description.abstract | Mechanical properties of cells have been shown to have a significant role in disease, as in many instances cell stiffness changes when a cell is no longer healthy. We present a high-throughput microfluidics-based approach that exploits the connection between travel time of a cell through a narrow passage and cell stiffness. The system resolves both cell travel time and relative cell diameter while retaining information on the cell level. We show that stiffer cells have longer transit times than less stiff ones and that cell size significantly influences travel times. Experiments with untreated HeLa cells and cells made compliant with latrunculin A and cytochalasin B further demonstrate that travel time is influenced by cell stiffness, with the compliant cells having faster transit time. | en_US |
dc.description.sponsorship | National Institutes of Health (U.S.) (NIH grant RC1 EB011187-02) | en_US |
dc.language.iso | en_US | |
dc.publisher | American Chemical Society (ACS) | en_US |
dc.relation.isversionof | http://dx.doi.org/10.1021/ac300264v | en_US |
dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
dc.source | PMC | en_US |
dc.title | Microfluidics-Based Assessment of Cell Deformability | en_US |
dc.type | Article | en_US |
dc.identifier.citation | Adamo, Andrea, Armon Sharei, Luigi Adamo, ByungKun Lee, Shirley Mao, and Klavs F. Jensen. “Microfluidics-Based Assessment of Cell Deformability.” Analytical Chemistry 84, no. 15 (August 7, 2012): 6438–6443. © 2012 American Chemical Society. | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
dc.contributor.department | Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science | en_US |
dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
dc.contributor.mitauthor | Adamo, Andrea | en_US |
dc.contributor.mitauthor | Sharei, Armon Reza | en_US |
dc.contributor.mitauthor | Lee, ByungKun | en_US |
dc.contributor.mitauthor | Mao, Shirley | en_US |
dc.contributor.mitauthor | Jensen, Klavs F. | en_US |
dc.relation.journal | Analytical Chemistry | en_US |
dc.eprint.version | Author's final manuscript | en_US |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
dspace.orderedauthors | Adamo, Andrea; Sharei, Armon; Adamo, Luigi; Lee, ByungKun; Mao, Shirley; Jensen, Klavs F. | en_US |
dc.identifier.orcid | https://orcid.org/0000-0001-7192-580X | |
dc.identifier.orcid | https://orcid.org/0000-0001-5262-2264 | |
mit.license | PUBLISHER_POLICY | en_US |
mit.metadata.status | Complete | |