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dc.contributor.authorXu, Cong
dc.contributor.authorFan, Zi Peng
dc.contributor.authorFogley, Rachel
dc.contributor.authorDiBiase, Anthony
dc.contributor.authorTrompouki, Eirini
dc.contributor.authorUnternaehrer, Juli
dc.contributor.authorXiong, Fengzhu
dc.contributor.authorTorregroza, Ingrid
dc.contributor.authorEvans, Todd
dc.contributor.authorMegason, Sean G.
dc.contributor.authorDaley, George Q.
dc.contributor.authorSchier, Alexander F.
dc.contributor.authorYoung, Richard A.
dc.contributor.authorZon, Leonard I.
dc.contributor.authorMuller, Patrick
dc.contributor.authorYoung, Richard A.
dc.date.accessioned2014-11-12T13:17:51Z
dc.date.available2014-11-12T13:17:51Z
dc.date.issued2012-03
dc.date.submitted2011-09
dc.identifier.issn15345807
dc.identifier.issn1878-1551
dc.identifier.urihttp://hdl.handle.net/1721.1/91520
dc.description.abstractIn mammalian embryonic stem cells, the acquisition of pluripotency is dependent on Nanog, but the in vivo analysis of Nanog has been hampered by its requirement for early mouse development. In an effort to examine the role of Nanog in vivo, we identified a zebrafish Nanog ortholog and found that its knockdown impaired endoderm formation. Genome-wide transcription analysis revealed that nanog-like morphants fail to develop the extraembryonic yolk syncytial layer (YSL), which produces Nodal, required for endoderm induction. We examined the genes that were regulated by Nanog-like and identified the homeobox gene mxtx2, which is both necessary and sufficient for YSL induction. Chromatin immunoprecipitation assays and genetic studies indicated that Nanog-like directly activates mxtx2, which, in turn, specifies the YSL lineage by directly activating YSL genes. Our study identifies a Nanog-like-Mxtx2-Nodal pathway and establishes a role for Nanog-like in regulating the formation of the extraembryonic tissue required for endoderm induction.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01 HG002668)en_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.devcel.2012.01.003en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceElsevieren_US
dc.titleNanog-like Regulates Endoderm Formation through the Mxtx2-Nodal Pathwayen_US
dc.typeArticleen_US
dc.identifier.citationXu, Cong, Zi Peng Fan, Patrick Muller, Rachel Fogley, Anthony DiBiase, Eirini Trompouki, Juli Unternaehrer, et al. “Nanog-Like Regulates Endoderm Formation through the Mxtx2-Nodal Pathway.” Developmental Cell 22, no. 3 (March 2012): 625–638. © 2012 Elsevier Inc.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Computational and Systems Biology Programen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorFan, Zi Pengen_US
dc.contributor.mitauthorYoung, Richard A.en_US
dc.relation.journalDevelopmental Cellen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsXu, Cong; Fan, Zi Peng; Muller, Patrick; Fogley, Rachel; DiBiase, Anthony; Trompouki, Eirini; Unternaehrer, Juli; Xiong, Fengzhu; Torregroza, Ingrid; Evans, Todd; Megason, Sean G.; Daley, George Q.; Schier, Alexander F.; Young, Richard A.; Zon, Leonard I.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-8855-8647
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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