Flow-Through Comb Electroporation Device for Delivery of Macromolecules

Author(s)
Adamo, Andrea; Arione, Alessandro; Sharei, Armon Reza; Jensen, Klavs F
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Abstract
We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (~95%) with low toxicity (cell viability ~85%) as well as rapid pore closure after electroporation. The activity of delivered molecules is also verified by silencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells. This simple, scalable approach to microfluidic, flow-through electroporation could facilitate the integration of electroporation modules within cell analysis devices that perform multiple operations.
Date issued
2012-12
URI
http://hdl.handle.net/1721.1/91542
Department
Massachusetts Institute of Technology. Department of Chemical Engineering; Koch Institute for Integrative Cancer Research at MIT
Journal
Analytical Chemistry
Publisher
American Chemical Society (ACS)
Citation
Adamo, Andrea, Alessandro Arione, Armon Sharei, and Klavs F. Jensen. “Flow-Through Comb Electroporation Device for Delivery of Macromolecules.” Analytical Chemistry 85, no. 3 (February 5, 2013): 1637–1641.
Version: Author's final manuscript
ISSN
0003-2700
1520-6882
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