| dc.contributor.author | Adamo, Andrea | |
| dc.contributor.author | Arione, Alessandro | |
| dc.contributor.author | Sharei, Armon Reza | |
| dc.contributor.author | Jensen, Klavs F | |
| dc.date.accessioned | 2014-11-13T18:24:42Z | |
| dc.date.available | 2014-11-13T18:24:42Z | |
| dc.date.issued | 2012-12 | |
| dc.date.submitted | 2012-10 | |
| dc.identifier.issn | 0003-2700 | |
| dc.identifier.issn | 1520-6882 | |
| dc.identifier.uri | http://hdl.handle.net/1721.1/91542 | |
| dc.description.abstract | We present a microfluidic electroporation device with a comb electrode layout fabricated in polydimethylsiloxane (PMDS) and glass. Characterization experiments with HeLa cells and fluorescent dextran show efficient delivery (~95%) with low toxicity (cell viability ~85%) as well as rapid pore closure after electroporation. The activity of delivered molecules is also verified by silencing RNA (siRNA) studies that demonstrate gene knockdown in GFP expressing cells. This simple, scalable approach to microfluidic, flow-through electroporation could facilitate the integration of electroporation modules within cell analysis devices that perform multiple operations. | en_US |
| dc.description.sponsorship | National Institutes of Health (U.S.) (Grant RC1 EB011187-02) | en_US |
| dc.description.sponsorship | National Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051) | en_US |
| dc.language.iso | en_US | |
| dc.publisher | American Chemical Society (ACS) | en_US |
| dc.relation.isversionof | http://dx.doi.org/10.1021/ac302887a | en_US |
| dc.rights | Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. | en_US |
| dc.source | PMC | en_US |
| dc.title | Flow-Through Comb Electroporation Device for Delivery of Macromolecules | en_US |
| dc.type | Article | en_US |
| dc.identifier.citation | Adamo, Andrea, Alessandro Arione, Armon Sharei, and Klavs F. Jensen. “Flow-Through Comb Electroporation Device for Delivery of Macromolecules.” Analytical Chemistry 85, no. 3 (February 5, 2013): 1637–1641. | en_US |
| dc.contributor.department | Massachusetts Institute of Technology. Department of Chemical Engineering | en_US |
| dc.contributor.department | Koch Institute for Integrative Cancer Research at MIT | en_US |
| dc.contributor.mitauthor | Adamo, Andrea | en_US |
| dc.contributor.mitauthor | Arione, Alessandro | en_US |
| dc.contributor.mitauthor | Sharei, Armon Reza | en_US |
| dc.contributor.mitauthor | Jensen, Klavs F. | en_US |
| dc.relation.journal | Analytical Chemistry | en_US |
| dc.eprint.version | Author's final manuscript | en_US |
| dc.type.uri | http://purl.org/eprint/type/JournalArticle | en_US |
| eprint.status | http://purl.org/eprint/status/PeerReviewed | en_US |
| dspace.orderedauthors | Adamo, Andrea; Arione, Alessandro; Sharei, Armon; Jensen, Klavs F. | en_US |
| dc.identifier.orcid | https://orcid.org/0000-0001-7192-580X | |
| mit.license | PUBLISHER_POLICY | en_US |
| mit.metadata.status | Complete | |
