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In vivo biosensing via tissue-localizable near-infrared-fluorescent single-walled carbon nanotubes

Author(s)
Iverson, Nicole M.; Barone, Paul W.; Shandell, Mia; Trudel, Laura J.; Sen, Selda; Sen, Fatih; Ivanov, Vsevolod; Atolia, Esha; Farias, Edgardo; McNicholas, Thomas P.; Reuel, Nigel; Parry, Nicola M. A.; Wogan, Gerald N.; Strano, Michael S.; ... Show more Show less
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Abstract
Single-walled carbon nanotubes are particularly attractive for biomedical applications, because they exhibit a fluorescent signal in a spectral region where there is minimal interference from biological media. Although single-walled carbon nanotubes have been used as highly sensitive detectors for various compounds, their use as in vivo biomarkers requires the simultaneous optimization of various parameters, including biocompatibility, molecular recognition, high fluorescence quantum efficiency and signal transduction. Here we show that a polyethylene glycol ligated copolymer stabilizes near-infrared-fluorescent single-walled carbon nanotubes sensors in solution, enabling intravenous injection into mice and the selective detection of local nitric oxide concentration with a detection limit of 1 µM. The half-life for liver retention is 4 h, with sensors clearing the lungs within 2 h after injection, thus avoiding a dominant route of in vivo nanotoxicology. After localization within the liver, it is possible to follow the transient inflammation using nitric oxide as a marker and signalling molecule. To this end, we also report a spatial-spectral imaging algorithm to deconvolute fluorescence intensity and spatial information from measurements. Finally, we demonstrate that alginate-encapsulated single-walled carbon nanotubes can function as implantable inflammation sensors for nitric oxide detection, with no intrinsic immune reactivity or other adverse response for more than 400 days.
Date issued
2013-11
URI
http://hdl.handle.net/1721.1/91579
Department
Massachusetts Institute of Technology. Center for Biomedical Innovation; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Division of Comparative Medicine
Journal
Nature Nanotechnology
Publisher
Nature Publishing Group
Citation
Iverson, Nicole M., Paul W. Barone, Mia Shandell, Laura J. Trudel, Selda Sen, Fatih Sen, Vsevolod Ivanov, et al. “In Vivo Biosensing via Tissue-Localizable Near-Infrared-Fluorescent Single-Walled Carbon Nanotubes.” Nature Nanotechnology 8, no. 11 (November 3, 2013): 873–880.
Version: Author's final manuscript
ISSN
1748-3387
1748-3395

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