Label-Free Imaging of Membrane Potential Using Membrane Electromotility
Author(s)Oh, Seungeun; Fang-Yen, Chris; Choi, Wonshik; Yaqoob, Zahid; Fu, Dan; Park, YongKeun; Dassari, Ramachandra R.; Feld, Michael S.; ... Show more Show less
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Electrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity.
DepartmentMassachusetts Institute of Technology. Department of Chemistry; Massachusetts Institute of Technology. Spectroscopy Laboratory
Oh, Seungeun, Christopher Fang-Yen, Wonshik Choi, Zahid Yaqoob, Dan Fu, YongKeun Park, Ramachandra R. Dassari, and Michael S. Feld. “Label-Free Imaging of Membrane Potential Using Membrane Electromotility.” Biophysical Journal 103, no. 1 (July 2012): 11–18. © 2012 Biophysical Society
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