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Label-Free Imaging of Membrane Potential Using Membrane Electromotility

Author(s)
Oh, Seungeun; Fang-Yen, Chris; Choi, Wonshik; Yaqoob, Zahid; Fu, Dan; Park, YongKeun; Dassari, Ramachandra R.; Feld, Michael S.; ... Show more Show less
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Abstract
Electrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity.
Date issued
2012-07
URI
http://hdl.handle.net/1721.1/91628
Department
Massachusetts Institute of Technology. Department of Chemistry; Massachusetts Institute of Technology. Spectroscopy Laboratory
Journal
Biophysical Journal
Publisher
Elsevier
Citation
Oh, Seungeun, Christopher Fang-Yen, Wonshik Choi, Zahid Yaqoob, Dan Fu, YongKeun Park, Ramachandra R. Dassari, and Michael S. Feld. “Label-Free Imaging of Membrane Potential Using Membrane Electromotility.” Biophysical Journal 103, no. 1 (July 2012): 11–18. © 2012 Biophysical Society
Version: Final published version
ISSN
00063495
1542-0086

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