Show simple item record

dc.contributor.authorOh, Seungeun
dc.contributor.authorFang-Yen, Chris
dc.contributor.authorChoi, Wonshik
dc.contributor.authorYaqoob, Zahid
dc.contributor.authorFu, Dan
dc.contributor.authorPark, YongKeun
dc.contributor.authorDassari, Ramachandra R.
dc.contributor.authorFeld, Michael S.
dc.date.accessioned2014-11-20T13:28:43Z
dc.date.available2014-11-20T13:28:43Z
dc.date.issued2012-07
dc.date.submitted2011-09
dc.identifier.issn00063495
dc.identifier.issn1542-0086
dc.identifier.urihttp://hdl.handle.net/1721.1/91628
dc.description.abstractElectrical activity may cause observable changes in a cell's structure in the absence of exogenous reporter molecules. In this work, we report a low-coherence interferometric microscopy technique that can detect an optical signal correlated with the membrane potential changes in individual mammalian cells without exogenous labels. By measuring milliradian-scale phase shifts in the transmitted light, we can detect changes in the cells' membrane potential. We find that the observed optical signals are due to membrane electromotility, which causes the cells to deform in response to the membrane potential changes. We demonstrate wide-field imaging of the propagation of electrical stimuli in gap-junction-coupled cell networks. Membrane electromotility-induced cell deformation may be useful as a reporter of electrical activity.en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (DBI-0754339)en_US
dc.description.sponsorshipNational Center for Research Resources (U.S.) (P41-RR02594-24)en_US
dc.description.sponsorshipHamamatsu Corporationen_US
dc.language.isoen_US
dc.publisherElsevieren_US
dc.relation.isversionofhttp://dx.doi.org/10.1016/j.bpj.2012.05.020en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceElsevieren_US
dc.titleLabel-Free Imaging of Membrane Potential Using Membrane Electromotilityen_US
dc.typeArticleen_US
dc.identifier.citationOh, Seungeun, Christopher Fang-Yen, Wonshik Choi, Zahid Yaqoob, Dan Fu, YongKeun Park, Ramachandra R. Dassari, and Michael S. Feld. “Label-Free Imaging of Membrane Potential Using Membrane Electromotility.” Biophysical Journal 103, no. 1 (July 2012): 11–18. © 2012 Biophysical Societyen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemistryen_US
dc.contributor.departmentMassachusetts Institute of Technology. Spectroscopy Laboratoryen_US
dc.contributor.mitauthorYaqoob, Zahiden_US
dc.contributor.mitauthorOh, Seungeunen_US
dc.contributor.mitauthorFang-Yen, Chrisen_US
dc.contributor.mitauthorChoi, Wonshiken_US
dc.contributor.mitauthorFu, Danen_US
dc.contributor.mitauthorPark, YongKeunen_US
dc.contributor.mitauthorDassari, Ramachandra R.en_US
dc.contributor.mitauthorFeld, Michael S.en_US
dc.relation.journalBiophysical Journalen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsOh, Seungeun; Fang-Yen, Christopher; Choi, Wonshik; Yaqoob, Zahid; Fu, Dan; Park, YongKeun; Dassari, Ramachandra R.; Feld, Michael S.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-4568-3218
dspace.mitauthor.errortrue
mit.licensePUBLISHER_POLICYen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record