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Noninvasive optical inhibition with a red-shifted microbial rhodopsin

Author(s)
Miri, Mitra L.; Busskamp, Volker; Young, Andrew; Ogawa, Masaaki; Ramanlal, Shreshtha B.; Forest, Craig R.; Chow, Brian Y.; Han, Xue; Lin, Yingxi; Roska, Botond; Cardin, Jessica A.; Chuong, Amy S.; Acker, Leah Christine; Henninger, Michael Alan; Kodandaramaiah, Suhasa Bangalo; Bandler, Rachel C.; Allen, Brian Douglas; Boyden, Edward Stuart; Sorensen, Andreas Toft; Klapoetke, Nathan Cao; Matthews, Gillian A.; Tye, Kay; ... Show more Show less
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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Abstract
Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light–induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.
Date issued
2014-07
URI
http://hdl.handle.net/1721.1/95967
Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; Massachusetts Institute of Technology. Department of Physics; McGovern Institute for Brain Research at MIT; Picower Institute for Learning and Memory; Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Journal
Nature Neuroscience
Publisher
Nature Publishing Group
Citation
Chuong, Amy S, Mitra L Miri, Volker Busskamp, Gillian A C Matthews, Leah C Acker, Andreas T Sorensen, Andrew Young, et al. “Noninvasive Optical Inhibition with a Red-Shifted Microbial Rhodopsin.” Nat Neurosci 17, no. 8 (July 6, 2014): 1123–1129.
Version: Author's final manuscript
ISSN
1097-6256
1546-1726

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