Noninvasive optical inhibition with a red-shifted microbial rhodopsin
Author(s)Miri, Mitra L.; Busskamp, Volker; Young, Andrew; Ogawa, Masaaki; Ramanlal, Shreshtha B.; Forest, Craig R.; Chow, Brian Y.; Han, Xue; Lin, Yingxi; Roska, Botond; Cardin, Jessica A.; Chuong, Amy S.; Acker, Leah Christine; Henninger, Michael Alan; Kodandaramaiah, Suhasa Bangalo; Bandler, Rachel C.; Allen, Brian Douglas; Boyden, Edward Stuart; Sorensen, Andreas Toft; Klapoetke, Nathan Cao; Matthews, Gillian A.; Tye, Kay; ... Show more Show less
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Optogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light–induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; Massachusetts Institute of Technology. Department of Physics; McGovern Institute for Brain Research at MIT; Picower Institute for Learning and Memory; Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Nature Publishing Group
Chuong, Amy S, Mitra L Miri, Volker Busskamp, Gillian A C Matthews, Leah C Acker, Andreas T Sorensen, Andrew Young, et al. “Noninvasive Optical Inhibition with a Red-Shifted Microbial Rhodopsin.” Nat Neurosci 17, no. 8 (July 6, 2014): 1123–1129.
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