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dc.contributor.authorMiri, Mitra L.
dc.contributor.authorBusskamp, Volker
dc.contributor.authorYoung, Andrew
dc.contributor.authorOgawa, Masaaki
dc.contributor.authorRamanlal, Shreshtha B.
dc.contributor.authorForest, Craig R.
dc.contributor.authorChow, Brian Y.
dc.contributor.authorHan, Xue
dc.contributor.authorLin, Yingxi
dc.contributor.authorRoska, Botond
dc.contributor.authorCardin, Jessica A.
dc.contributor.authorChuong, Amy S.
dc.contributor.authorAcker, Leah Christine
dc.contributor.authorHenninger, Michael Alan
dc.contributor.authorKodandaramaiah, Suhasa Bangalo
dc.contributor.authorBandler, Rachel C.
dc.contributor.authorAllen, Brian Douglas
dc.contributor.authorBoyden, Edward Stuart
dc.contributor.authorSorensen, Andreas Toft
dc.contributor.authorKlapoetke, Nathan Cao
dc.contributor.authorMatthews, Gillian A.
dc.contributor.authorTye, Kay
dc.date.accessioned2015-03-11T20:42:25Z
dc.date.available2015-03-11T20:42:25Z
dc.date.issued2014-07
dc.date.submitted2014-02
dc.identifier.issn1097-6256
dc.identifier.issn1546-1726
dc.identifier.urihttp://hdl.handle.net/1721.1/95967
dc.description.abstractOptogenetic inhibition of the electrical activity of neurons enables the causal assessment of their contributions to brain functions. Red light penetrates deeper into tissue than other visible wavelengths. We present a red-shifted cruxhalorhodopsin, Jaws, derived from Haloarcula (Halobacterium) salinarum (strain Shark) and engineered to result in red light–induced photocurrents three times those of earlier silencers. Jaws exhibits robust inhibition of sensory-evoked neural activity in the cortex and results in strong light responses when used in retinas of retinitis pigmentosa model mice. We also demonstrate that Jaws can noninvasively mediate transcranial optical inhibition of neurons deep in the brains of awake mice. The noninvasive optogenetic inhibition opened up by Jaws enables a variety of important neuroscience experiments and offers a powerful general-use chloride pump for basic and applied neuroscience.en_US
dc.description.sponsorshipMcGovern Institute for Brain Research at MIT (Razin Fellowship)en_US
dc.description.sponsorshipUnited States. Defense Advanced Research Projects Agency. Living Foundries Program (HR0011-12-C-0068)en_US
dc.description.sponsorshipHarvard-MIT Joint Research Grants Program in Basic Neuroscienceen_US
dc.description.sponsorshipHuman Frontier Science Program (Strasbourg, France)en_US
dc.description.sponsorshipInstitution of Engineering and Technology (A. F. Harvey Prize)en_US
dc.description.sponsorshipMcGovern Institute for Brain Research at MIT. Neurotechnology (MINT) Programen_US
dc.description.sponsorshipNew York Stem Cell Foundation (Robertson Investigator Award)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (New Innovator Award 1DP2OD002002)en_US
dc.description.sponsorshipNational Institute of General Medical Sciences (U.S.) (EUREKA Award 1R01NS075421)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 1R01DA029639)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 1RC1MH088182)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant 1R01NS067199)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Career Award CBET 1053233)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Grant EFRI0835878)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (Grant DMS0848804)en_US
dc.description.sponsorshipSociety for Neuroscience (Research Award for Innovation in Neuroscience)en_US
dc.description.sponsorshipWallace H. Coulter Foundationen_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (RO1 MH091220-01)en_US
dc.description.sponsorshipWhitehall Foundationen_US
dc.description.sponsorshipEsther A. & Joseph Klingenstein Fund, Inc.en_US
dc.description.sponsorshipJPB Foundationen_US
dc.description.sponsorshipPIIF Fundingen_US
dc.description.sponsorshipNational Institute of Mental Health (U.S.) (R01-MH102441-01)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (DP2-OD-017366-01)en_US
dc.description.sponsorshipMassachusetts Institute of Technology. Simons Center for the Social Brainen_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/nn.3752en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleNoninvasive optical inhibition with a red-shifted microbial rhodopsinen_US
dc.typeArticleen_US
dc.identifier.citationChuong, Amy S, Mitra L Miri, Volker Busskamp, Gillian A C Matthews, Leah C Acker, Andreas T Sorensen, Andrew Young, et al. “Noninvasive Optical Inhibition with a Red-Shifted Microbial Rhodopsin.” Nat Neurosci 17, no. 8 (July 6, 2014): 1123–1129.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Brain and Cognitive Sciencesen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Physicsen_US
dc.contributor.departmentMcGovern Institute for Brain Research at MITen_US
dc.contributor.departmentPicower Institute for Learning and Memoryen_US
dc.contributor.departmentProgram in Media Arts and Sciences (Massachusetts Institute of Technology)en_US
dc.contributor.mitauthorChuong, Amy S.en_US
dc.contributor.mitauthorAcker, Leah Christineen_US
dc.contributor.mitauthorHenninger, Michael Alanen_US
dc.contributor.mitauthorKodandaramaiah, Suhasa Bangaloen_US
dc.contributor.mitauthorOgawa, Masaakien_US
dc.contributor.mitauthorBandler, Rachel C.en_US
dc.contributor.mitauthorAllen, Brian Douglasen_US
dc.contributor.mitauthorBoyden, Edward Stuarten_US
dc.contributor.mitauthorSorensen, Andreas Toften_US
dc.contributor.mitauthorYoung, Andrewen_US
dc.contributor.mitauthorKlapoetke, Nathan Caoen_US
dc.contributor.mitauthorLin, Yingxien_US
dc.contributor.mitauthorMatthews, Gillian A.en_US
dc.contributor.mitauthorTye, Kayen_US
dc.relation.journalNature Neuroscienceen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsChuong, Amy S; Miri, Mitra L; Busskamp, Volker; Matthews, Gillian A C; Acker, Leah C; Sorensen, Andreas T; Young, Andrew; Klapoetke, Nathan C; Henninger, Mike A; Kodandaramaiah, Suhasa B; Ogawa, Masaaki; Ramanlal, Shreshtha B; Bandler, Rachel C; Allen, Brian D; Forest, Craig R; Chow, Brian Y; Han, Xue; Lin, Yingxi; Tye, Kay M; Roska, Botond; Cardin, Jessica A; Boyden, Edward Sen_US
dc.identifier.orcidhttps://orcid.org/0000-0003-2218-7489
dc.identifier.orcidhttps://orcid.org/0000-0001-6754-0333
dc.identifier.orcidhttps://orcid.org/0000-0002-7002-1275
dc.identifier.orcidhttps://orcid.org/0000-0002-5680-2630
dc.identifier.orcidhttps://orcid.org/0000-0002-5534-6020
dc.identifier.orcidhttps://orcid.org/0000-0001-9969-4281
dc.identifier.orcidhttps://orcid.org/0000-0002-0419-3351
dspace.mitauthor.errortrue
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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