The Role of Transcription in the Activation of a Drosophila Amplification Origin
Name
Orr-Weaver_The role.pdf
Size
1.04 MB
Format
Adobe PDF
Checksum (MD5)
ad92203f57956750477a4faeb6f33d40
Author(s)
Hua, Brian
Li, Sharon
Orr-Weaver, Terry
Date Issued
October 2014
Journal
G3: Genes-Genomes-Genetics
Publisher
Genetics Society of America, The
Citation
Hua, B. L., S. Li, and T. L. Orr-Weaver. “The Role of Transcription in the Activation of a Drosophila Amplification Origin.” G3: Genes-Genomes-Genetics 4, no. 12 (October 14, 2014): 2403–2408.
Version
Final published version
Abstract
The mechanisms that underlie metazoan DNA replication initiation, especially the connection between transcription and replication origin activation, are not well understood. To probe the role of transcription in origin activation, we exploited a specific replication origin in Drosophila melanogaster follicle cells, ori62, which coincides with the yellow-g2 transcription unit and exhibits transcription-dependent origin firing. Within a 10-kb genomic fragment that contains ori62 and is sufficient for amplification, RNA-sequencing analysis revealed that all detected RNAs mapped solely to the yellow-g2 gene. To determine whether transcription is required in cis for ori62 firing, we generated a set of tagged yellow-g2 transgenes in which we could prevent local transcription across ori62 by deletions in the yellow-g2 promoter. Surprisingly, inhibition of yellow-g2 transcription by promoter deletions did not affect ori62 firing. Our results reveal that transcription in cis is not required for ori62 firing, raising the possibility that a trans-acting factor is required specifically for the activation of ori62. This finding illustrates that a diversity of mechanisms can be used in the regulation of metazoan DNA replication initiation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution Unported License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1534/g3.114.014050
