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dc.contributor.authorOrr-Weaver, Terry L.
dc.contributor.authorHua, Brian
dc.contributor.authorLi, Sharon
dc.date.accessioned2015-04-21T18:55:25Z
dc.date.available2015-04-21T18:55:25Z
dc.date.issued2014-10
dc.date.submitted2014-08
dc.identifier.issn2160-1836
dc.identifier.urihttp://hdl.handle.net/1721.1/96690
dc.description.abstractThe mechanisms that underlie metazoan DNA replication initiation, especially the connection between transcription and replication origin activation, are not well understood. To probe the role of transcription in origin activation, we exploited a specific replication origin in Drosophila melanogaster follicle cells, ori62, which coincides with the yellow-g2 transcription unit and exhibits transcription-dependent origin firing. Within a 10-kb genomic fragment that contains ori62 and is sufficient for amplification, RNA-sequencing analysis revealed that all detected RNAs mapped solely to the yellow-g2 gene. To determine whether transcription is required in cis for ori62 firing, we generated a set of tagged yellow-g2 transgenes in which we could prevent local transcription across ori62 by deletions in the yellow-g2 promoter. Surprisingly, inhibition of yellow-g2 transcription by promoter deletions did not affect ori62 firing. Our results reveal that transcription in cis is not required for ori62 firing, raising the possibility that a trans-acting factor is required specifically for the activation of ori62. This finding illustrates that a diversity of mechanisms can be used in the regulation of metazoan DNA replication initiation.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM57960)en_US
dc.language.isoen_US
dc.publisherGenetics Society of America, Theen_US
dc.relation.isversionofhttp://dx.doi.org/10.1534/g3.114.014050en_US
dc.rightsCreative Commons Attribution Unported Licenseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/en_US
dc.sourceG3en_US
dc.titleThe Role of Transcription in the Activation of a Drosophila Amplification Originen_US
dc.typeArticleen_US
dc.identifier.citationHua, B. L., S. Li, and T. L. Orr-Weaver. “The Role of Transcription in the Activation of a Drosophila Amplification Origin.” G3: Genes-Genomes-Genetics 4, no. 12 (October 14, 2014): 2403–2408.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.departmentWhitehead Institute for Biomedical Researchen_US
dc.contributor.mitauthorOrr-Weaver, Terry L.en_US
dc.contributor.mitauthorHua, Brianen_US
dc.relation.journalG3: Genes-Genomes-Geneticsen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsHua, B. L.; Li, S.; Orr-Weaver, T. L.en_US
dc.identifier.orcidhttps://orcid.org/0000-0002-7934-111X
dc.identifier.orcidhttps://orcid.org/0000-0002-7580-3399
mit.licensePUBLISHER_CCen_US


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