Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue
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The existence of nonannotated protein-coding human short open reading frames (sORFs) has been revealed through the direct detection of their sORF-encoded polypeptide (SEP) products. The discovery of novel SEPs increases the size of the genome and the proteome and provides insights into the molecular biology of mammalian cells, such as the prevalent usage of non-AUG start codons. Through modifications of the existing SEP-discovery workflow, we discover an additional 195 SEPs in K562 cells and extend this methodology to identify novel human SEPs in additional cell lines and human tissue for a final tally of 237 new SEPs. These results continue to expand the human genome and proteome and demonstrate that SEPs are a ubiquitous class of nonannotated polypeptides that require further investigation.
Date issued
2014-03Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer ScienceJournal
Journal of Proteome Research
Publisher
American Chemical Society (ACS)
Citation
Ma, Jiao, Carl C. Ward, Irwin Jungreis, Sarah A. Slavoff, Adam G. Schwaid, John Neveu, Bogdan A. Budnik, Manolis Kellis, and Alan Saghatelian. “Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue.” Journal of Proteome Research 13, no. 3 (March 7, 2014): 1757–1765.© 2014 American Chemical Society.
Version: Final published version
ISSN
1535-3893
1535-3907