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Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue

dc.contributor.authorMa, Jiao
dc.contributor.authorWard, Carl C.
dc.contributor.authorJungreis, Irwin
dc.contributor.authorSlavoff, Sarah A.
dc.contributor.authorSchwaid, Adam G.
dc.contributor.authorNeveu, John
dc.contributor.authorBudnik, Bogdan A.
dc.contributor.authorKellis, Manolis
dc.contributor.authorSaghatelian, Alan
dc.date.accessioned2015-04-23T15:11:27Z
dc.date.available2015-04-23T15:11:27Z
dc.date.issued2014-03
dc.identifier.issn1535-3893
dc.identifier.issn1535-3907
dc.identifier.urihttp://hdl.handle.net/1721.1/96734
dc.description.abstractThe existence of nonannotated protein-coding human short open reading frames (sORFs) has been revealed through the direct detection of their sORF-encoded polypeptide (SEP) products. The discovery of novel SEPs increases the size of the genome and the proteome and provides insights into the molecular biology of mammalian cells, such as the prevalent usage of non-AUG start codons. Through modifications of the existing SEP-discovery workflow, we discover an additional 195 SEPs in K562 cells and extend this methodology to identify novel human SEPs in additional cell lines and human tissue for a final tally of 237 new SEPs. These results continue to expand the human genome and proteome and demonstrate that SEPs are a ubiquitous class of nonannotated polypeptides that require further investigation.en_US
dc.description.sponsorshipNational Institute on Deafness and Other Communication Disorders (U.S.) (National Research Service Award postdoctoral fellowship (1F32GM099408-01))en_US
dc.description.sponsorshipNational Institute of General Medical Sciences (U.S.) (grant R01GM102491)en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (NIH U41 HG007234)en_US
dc.description.sponsorshipNational Science Foundation (U.S.) (NSF CAREER 0644282)en_US
dc.language.isoen_US
dc.publisherAmerican Chemical Society (ACS)en_US
dc.relation.isversionofhttp://dx.doi.org/10.1021/pr401280wen_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourceAmerican Chemical Societyen_US
dc.titleDiscovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissueen_US
dc.typeArticleen_US
dc.identifier.citationMa, Jiao, Carl C. Ward, Irwin Jungreis, Sarah A. Slavoff, Adam G. Schwaid, John Neveu, Bogdan A. Budnik, Manolis Kellis, and Alan Saghatelian. “Discovery of Human sORF-Encoded Polypeptides (SEPs) in Cell Lines and Tissue.” Journal of Proteome Research 13, no. 3 (March 7, 2014): 1757–1765.© 2014 American Chemical Society.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratoryen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.mitauthorJungreis, Irwinen_US
dc.contributor.mitauthorKellis, Manolisen_US
dc.relation.journalJournal of Proteome Researchen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsMa, Jiao; Ward, Carl C.; Jungreis, Irwin; Slavoff, Sarah A.; Schwaid, Adam G.; Neveu, John; Budnik, Bogdan A.; Kellis, Manolis; Saghatelian, Alanen_US
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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