Target specificity of the CRISPR-Cas9 system

Author(s)
Wu, Xuebing; Kriz, Andrea J.; Sharp, Phillip A.
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Abstract
The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.
Date issued
2014-08
URI
http://hdl.handle.net/1721.1/96803
Department
Massachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Journal
Quantitative Biology
Publisher
Springer-Verlag
Citation
Wu, Xuebing, Andrea J. Kriz, and Phillip A. Sharp. “Target Specificity of the CRISPR-Cas9 System.” Quantitative Biology 2, no. 2 (June 2014): 59–70.
Version: Author's final manuscript
ISSN
2095-4689
2095-4697
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