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dc.contributor.authorPini, Francesco
dc.contributor.authorFrage, Benjamin
dc.contributor.authorFerri, Lorenzo
dc.contributor.authorDe Nisco, Nicole J.
dc.contributor.authorMohapatra, Saswat S.
dc.contributor.authorTaddei, Lucilla
dc.contributor.authorFioravanti, Antonella
dc.contributor.authorDewitte, Frederique
dc.contributor.authorGalardini, Marco
dc.contributor.authorBrilli, Matteo
dc.contributor.authorVilleret, Vincent
dc.contributor.authorBazzicalupo, Marco
dc.contributor.authorMengoni, Alessio
dc.contributor.authorWalker, Graham C.
dc.contributor.authorBecker, Anke
dc.contributor.authorBiondi, Emanuele G.
dc.date.accessioned2015-04-28T19:28:29Z
dc.date.available2015-04-28T19:28:29Z
dc.date.issued2013-08
dc.date.submitted2013-07
dc.identifier.issn0950382X
dc.identifier.issn1365-2958
dc.identifier.urihttp://hdl.handle.net/1721.1/96829
dc.description.abstractSinorhizobium meliloti is a soil bacterium that invades the root nodules it induces on Medicago sativa, whereupon it undergoes an alteration of its cell cycle and differentiates into nitrogen-fixing, elongated and polyploid bacteroid with higher membrane permeability. In Caulobacter crescentus, a related alphaproteobacterium, the principal cell cycle regulator, CtrA, is inhibited by the phosphorylated response regulator DivK. The phosphorylation of DivK depends on the histidine kinase DivJ, while PleC is the principal phosphatase for DivK. Despite the importance of the DivJ in C. crescentus, the mechanistic role of this kinase has never been elucidated in other Alphaproteobacteria. We show here that the histidine kinases DivJ together with CbrA and PleC participate in a complex phosphorylation system of the essential response regulator DivK in S. meliloti. In particular, DivJ and CbrA are involved in DivK phosphorylation and in turn CtrA inactivation, thereby controlling correct cell cycle progression and the integrity of the cell envelope. In contrast, the essential PleC presumably acts as a phosphatase of DivK. Interestingly, we found that a DivJ mutant is able to elicit nodules and enter plant cells, but fails to establish an effective symbiosis suggesting that proper envelope and/or low CtrA levels are required for symbiosis.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant GM31010)en_US
dc.language.isoen_US
dc.publisherWiley Blackwellen_US
dc.relation.isversionofhttp://dx.doi.org/10.1111/mmi.12347en_US
dc.rightsCreative Commons Attribution-Noncommercial-Share Alikeen_US
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/en_US
dc.sourcePMCen_US
dc.titleThe DivJ, CbrA and PleC system controls DivK phosphorylation and symbiosis in Sinorhizobium melilotien_US
dc.typeArticleen_US
dc.identifier.citationPini, Francesco, Benjamin Frage, Lorenzo Ferri, Nicole J. De Nisco, Saswat S. Mohapatra, Lucilla Taddei, Antonella Fioravanti, et al. “ The DivJ, CbrA and PleC System Controls DivK Phosphorylation and Symbiosis in Sinorhizobium Meliloti .” Molecular Microbiology (August 19, 2013): n/a–n/a.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biologyen_US
dc.contributor.mitauthorDe Nisco, Nicole J.en_US
dc.contributor.mitauthorWalker, Graham C.en_US
dc.relation.journalMolecular Microbiologyen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsPini, Francesco; Frage, Benjamin; Ferri, Lorenzo; De Nisco, Nicole J.; Mohapatra, Saswat S.; Taddei, Lucilla; Fioravanti, Antonella; Dewitte, Frederique; Galardini, Marco; Brilli, Matteo; Villeret, Vincent; Bazzicalupo, Marco; Mengoni, Alessio; Walker, Graham C.; Becker, Anke; Biondi, Emanuele G.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7243-8261
dc.identifier.orcidhttps://orcid.org/0000-0002-7670-5301
mit.licenseOPEN_ACCESS_POLICYen_US


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