Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells
Author(s)Trifonova, Radiana; Jhunjhunwala, Siddharth; Hartoularos, George C.; Eyerman, Alexandra T.; Lytton-Jean, Abigail K. R.; Angin, Mathieu; Sharma, Siddhartha; Poceviciute, Roberta; Mao, Shirley; Heimann, Megan; Liu, Sophia; Talkar, Tanya; Khan, Omar F.; Addo, Marylyn M.; von Andrian, Ulrich H.; Langer, Robert; Lieberman, Judy; Jensen, Klavs F.; Sharei, Armon Reza; Anderson, Daniel Griffith; ... Show more Show less
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Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.
DepartmentDavid H. Koch Institute for Integrative Cancer Research at MIT; Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Ragon Institute of MGH, MIT and Harvard
Public Library of Science
Sharei, Armon, Radiana Trifonova, Siddharth Jhunjhunwala, George C. Hartoularos, Alexandra T. Eyerman, Abigail Lytton-Jean, Mathieu Angin, et al. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” Edited by Philip A Stumbles. PLOS ONE 10, no. 4 (April 13, 2015): e0118803.
Final published version