Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

Author(s)
Trifonova, Radiana; Jhunjhunwala, Siddharth; Hartoularos, George C.; Eyerman, Alexandra T.; Lytton-Jean, Abigail K. R.; ... Show more
Thumbnail
DownloadSharei-2015-Ex Vivo Cytosolic De.pdf (1.372Mb)
PUBLISHER_CC
Terms of use
Metadata
Show full item record
Abstract
Intracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.
Date issued
2015-04
URI
http://hdl.handle.net/1721.1/97117
Department
Massachusetts Institute of Technology. Department of Chemical Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Ragon Institute of MGH, MIT and Harvard; Koch Institute for Integrative Cancer Research at MIT
Journal
PLOS ONE
Publisher
Public Library of Science
Citation
Sharei, Armon, Radiana Trifonova, Siddharth Jhunjhunwala, George C. Hartoularos, Alexandra T. Eyerman, Abigail Lytton-Jean, Mathieu Angin, et al. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” Edited by Philip A Stumbles. PLOS ONE 10, no. 4 (April 13, 2015): e0118803.
Version: Final published version
ISSN
1932-6203
Collections