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Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells

dc.contributor.authorTrifonova, Radiana
dc.contributor.authorJhunjhunwala, Siddharth
dc.contributor.authorHartoularos, George C.
dc.contributor.authorEyerman, Alexandra T.
dc.contributor.authorLytton-Jean, Abigail K. R.
dc.contributor.authorAngin, Mathieu
dc.contributor.authorSharma, Siddhartha
dc.contributor.authorPoceviciute, Roberta
dc.contributor.authorMao, Shirley
dc.contributor.authorHeimann, Megan
dc.contributor.authorLiu, Sophia
dc.contributor.authorTalkar, Tanya
dc.contributor.authorKhan, Omar F.
dc.contributor.authorAddo, Marylyn M.
dc.contributor.authorvon Andrian, Ulrich H.
dc.contributor.authorLanger, Robert
dc.contributor.authorLieberman, Judy
dc.contributor.authorJensen, Klavs F.
dc.contributor.authorSharei, Armon Reza
dc.contributor.authorAnderson, Daniel Griffith
dc.date.accessioned2015-05-29T15:01:49Z
dc.date.available2015-05-29T15:01:49Z
dc.date.issued2015-04
dc.date.submitted2014-11
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/1721.1/97117
dc.description.abstractIntracellular delivery of biomolecules, such as proteins and siRNAs, into primary immune cells, especially resting lymphocytes, is a challenge. Here we describe the design and testing of microfluidic intracellular delivery systems that cause temporary membrane disruption by rapid mechanical deformation of human and mouse immune cells. Dextran, antibody and siRNA delivery performance is measured in multiple immune cell types and the approach’s potential to engineer cell function is demonstrated in HIV infection studies.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant R01GM101420-01A1)en_US
dc.description.sponsorshipKathy and Curt Marble Cancer Research Funden_US
dc.description.sponsorshipRagon Institute of MGH, MIT and Harvarden_US
dc.description.sponsorshipNational Cancer Institute (U.S.) (Cancer Center Support (Core) Grant P30-CA14051)en_US
dc.language.isoen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1371/journal.pone.0118803en_US
dc.rightsCreative Commons Attributionen_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_US
dc.sourcePublic Library of Scienceen_US
dc.titleEx Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cellsen_US
dc.typeArticleen_US
dc.identifier.citationSharei, Armon, Radiana Trifonova, Siddharth Jhunjhunwala, George C. Hartoularos, Alexandra T. Eyerman, Abigail Lytton-Jean, Mathieu Angin, et al. “Ex Vivo Cytosolic Delivery of Functional Macromolecules to Immune Cells.” Edited by Philip A Stumbles. PLOS ONE 10, no. 4 (April 13, 2015): e0118803.en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Chemical Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentRagon Institute of MGH, MIT and Harvarden_US
dc.contributor.departmentKoch Institute for Integrative Cancer Research at MITen_US
dc.contributor.mitauthorSharei, Armon Rezaen_US
dc.contributor.mitauthorJhunjhunwala, Siddharthen_US
dc.contributor.mitauthorHartoularos, George C.en_US
dc.contributor.mitauthorEyerman, Alexandra T.en_US
dc.contributor.mitauthorLytton-Jean, Abigail K. R.en_US
dc.contributor.mitauthorPoceviciute, Robertaen_US
dc.contributor.mitauthorMao, Shirleyen_US
dc.contributor.mitauthorHeimann, Meganen_US
dc.contributor.mitauthorLiu, Sophiaen_US
dc.contributor.mitauthorTalkar, Tanyaen_US
dc.contributor.mitauthorKhan, Omar F.en_US
dc.contributor.mitauthorAnderson, Daniel Griffithen_US
dc.contributor.mitauthorLanger, Roberten_US
dc.contributor.mitauthorJensen, Klavs F.en_US
dc.relation.journalPLOS ONEen_US
dc.eprint.versionFinal published versionen_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSharei, Armon; Trifonova, Radiana; Jhunjhunwala, Siddharth; Hartoularos, George C.; Eyerman, Alexandra T.; Lytton-Jean, Abigail; Angin, Mathieu; Sharma, Siddhartha; Poceviciute, Roberta; Mao, Shirley; Heimann, Megan; Liu, Sophia; Talkar, Tanya; Khan, Omar F.; Addo, Marylyn; von Andrian, Ulrich H.; Anderson, Daniel G.; Langer, Robert; Lieberman, Judy; Jensen, Klavs F.en_US
dc.identifier.orcidhttps://orcid.org/0000-0001-5629-4798
dc.identifier.orcidhttps://orcid.org/0000-0001-7192-580X
dc.identifier.orcidhttps://orcid.org/0000-0003-3811-2369
dc.identifier.orcidhttps://orcid.org/0000-0001-8046-2288
dc.identifier.orcidhttps://orcid.org/0000-0003-4255-0492
dspace.mitauthor.errortrue
mit.licensePUBLISHER_CCen_US
mit.metadata.statusComplete


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