Microfluidic probe for single-cell lysis and analysis in adherent tissue culture
Author(s)Sarkar, Aniruddh; Kolitz, Sarah; Lauffenburger, Douglas A.; Han, Jongyoon
Microfluidic probe for single-cell analysis in adherent tissue culture
MetadataShow full item record
Single-cell analysis provides information critical to understanding key disease processes that are characterized by significant cellular heterogeneity. Few current methods allow single-cell measurement without removing cells from the context of interest, which not only destroys contextual information but also may perturb the process under study. Here we present a microfluidic probe that lyses single adherent cells from standard tissue culture and captures the contents to perform single-cell biochemical assays. We use this probe to measure kinase and housekeeping protein activities, separately or simultaneously, from single human hepatocellular carcinoma cells in adherent culture. This tool has the valuable ability to perform measurements that clarify connections between extracellular context, signals and responses, especially in cases where only a few cells exhibit a characteristic of interest.
DepartmentMassachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Massachusetts Institute of Technology. Research Laboratory of Electronics
Nature Publishing Group
Sarkar, Aniruddh, Sarah Kolitz, Douglas A. Lauffenburger, and Jongyoon Han. “Microfluidic Probe for Single-Cell Analysis in Adherent Tissue Culture.” Nat Comms 5 (March 5, 2014).
Author's final manuscript