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dc.contributor.authorSarkar, Aniruddh
dc.contributor.authorKolitz, Sarah
dc.contributor.authorLauffenburger, Douglas A.
dc.contributor.authorHan, Jongyoon
dc.date.accessioned2015-10-27T16:32:54Z
dc.date.available2015-10-27T16:32:54Z
dc.date.issued2014-03
dc.date.submitted2013-07
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/1721.1/99477
dc.description.abstractSingle-cell analysis provides information critical to understanding key disease processes that are characterized by significant cellular heterogeneity. Few current methods allow single-cell measurement without removing cells from the context of interest, which not only destroys contextual information but also may perturb the process under study. Here we present a microfluidic probe that lyses single adherent cells from standard tissue culture and captures the contents to perform single-cell biochemical assays. We use this probe to measure kinase and housekeeping protein activities, separately or simultaneously, from single human hepatocellular carcinoma cells in adherent culture. This tool has the valuable ability to perform measurements that clarify connections between extracellular context, signals and responses, especially in cases where only a few cells exhibit a characteristic of interest.en_US
dc.description.sponsorshipNational Institutes of Health (U.S.) (Grant P50-GM068762)en_US
dc.description.sponsorshipUnited States. Army Research Office (Institute for Collaborative Biotechnologies Grant W911NF-09-0001)en_US
dc.language.isoen_US
dc.publisherNature Publishing Groupen_US
dc.relation.isversionofhttp://dx.doi.org/10.1038/ncomms4421en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.sourcePMCen_US
dc.titleMicrofluidic probe for single-cell lysis and analysis in adherent tissue cultureen_US
dc.title.alternativeMicrofluidic probe for single-cell analysis in adherent tissue cultureen_US
dc.typeArticleen_US
dc.identifier.citationSarkar, Aniruddh, Sarah Kolitz, Douglas A. Lauffenburger, and Jongyoon Han. “Microfluidic Probe for Single-Cell Analysis in Adherent Tissue Culture.” Nat Comms 5 (March 5, 2014).en_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Biological Engineeringen_US
dc.contributor.departmentMassachusetts Institute of Technology. Department of Electrical Engineering and Computer Scienceen_US
dc.contributor.departmentMassachusetts Institute of Technology. Research Laboratory of Electronicsen_US
dc.contributor.mitauthorSarkar, Aniruddhen_US
dc.contributor.mitauthorKolitz, Sarahen_US
dc.contributor.mitauthorLauffenburger, Douglas A.en_US
dc.contributor.mitauthorHan, Jongyoonen_US
dc.relation.journalNature Communicationsen_US
dc.eprint.versionAuthor's final manuscripten_US
dc.type.urihttp://purl.org/eprint/type/JournalArticleen_US
eprint.statushttp://purl.org/eprint/status/PeerRevieweden_US
dspace.orderedauthorsSarkar, Aniruddh; Kolitz, Sarah; Lauffenburger, Douglas A.; Han, Jongyoonen_US
dc.identifier.orcidhttps://orcid.org/0000-0001-7215-1439
mit.licensePUBLISHER_POLICYen_US
mit.metadata.statusComplete


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