Principles of genetic circuit design
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Voight_Principles of.pdf
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707.32 KB
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Adobe PDF
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Author(s)
Brophy, Jennifer Ann
Voigt, Christopher A.
Date Issued
April 2014
Journal
Nature Methods
Publisher
Nature Publishing Group
Citation
Brophy, Jennifer A N, and Christopher A Voigt. “Principles of Genetic Circuit Design.” Nat Meth 11, no. 5 (April 29, 2014): 508–520.
Version
Author's final manuscript
Abstract
Cells navigate environments, communicate and build complex patterns by initiating gene expression in response to specific signals. Engineers seek to harness this capability to program cells to perform tasks or create chemicals and materials that match the complexity seen in nature. This Review describes new tools that aid the construction of genetic circuits. Circuit dynamics can be influenced by the choice of regulators and changed with expression 'tuning knobs'. We collate the failure modes encountered when assembling circuits, quantify their impact on performance and review mitigation efforts. Finally, we discuss the constraints that arise from circuits having to operate within a living cell. Collectively, better tools, well-characterized parts and a comprehensive understanding of how to compose circuits are leading to a breakthrough in the ability to program living cells for advanced applications, from living therapeutics to the atomic manufacturing of functional materials.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Synthetic Biology Center
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/nmeth.2926
