Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy
Author(s)
Prevedel, Robert; Yoon, Young-Gyu; Hoffmann, Maximilian; Pak, Nikita; Wetzstein, Gordon; Kato, Saul; Schrödel, Tina; Raskar, Ramesh; Zimmer, Manuel; Boyden, Edward Stuart; Vaziri, Alipasha; ... Show more Show less
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High-speed, large-scale three-dimensional (3D) imaging of neuronal activity poses a major challenge in neuroscience. Here we demonstrate simultaneous functional imaging of neuronal activity at single-neuron resolution in an entire Caenorhabditis elegans and in larval zebrafish brain. Our technique captures the dynamics of spiking neurons in volumes of ~700 μm × 700 μm × 200 μm at 20 Hz. Its simplicity makes it an attractive tool for high-speed volumetric calcium imaging.
Date issued
2014-05Department
Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Media Laboratory; McGovern Institute for Brain Research at MITJournal
Nature Methods
Publisher
Springer Nature
Citation
Prevedel, Robert, Young-Gyu Yoon, Maximilian Hoffmann, Nikita Pak, Gordon Wetzstein, Saul Kato, Tina Schrödel, Ramesh Raskar, Manuel Zimmer, Edward S. Boyden, and Alipasha Vaziri. “Simultaneous Whole-Animal 3D Imaging of Neuronal Activity Using Light-Field Microscopy.” Nature Methods 11, no. 7 (May 18, 2014): 727–730.
Version: Author's final manuscript
ISSN
1548-7091
1548-7105