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Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4
(United States National Academy of Sciences, 2009-04)
Ectopic expression of defined transcription factors can reprogram somatic cells to induced pluripotent stem (iPS) cells, but the utility of iPS cells is hampered by the use of viral delivery systems. Small molecules offer ...
Technical Challenges in Using Human Induced Pluripotent Stem Cells to Model Disease
(Elsevier, 2009-12)
Reprogramming of human somatic cells uses readily accessible tissue, such as skin or blood, to generate embryonic-like induced pluripotent stem cells (iPSCs). This procedure has been applied to somatic cells from patients ...
Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function
(National Academy of Sciences, 2009-02)
Mutations of MECP2 (Methyl-CpG Binding Protein 2) cause Rett syndrome. As a chromatin-associated multifunctional protein, how MeCP2 integrates external signals and regulates neuronal function remain unclear. Although ...
Analysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET
(BioMed Central Ltd, 2008-08)
Background: Genome-wide approaches have begun to reveal the transcriptional networks responsible for pluripotency in embryonic stem (ES) cells. Chromatin Immunoprecipitation (ChIP) followed either by hybridization to a ...
TCRβ Feedback Signals Inhibit the Coupling of Recombinationally Accessible Vβ14 Segments with DJβ Complexes
(American Association of Immunologists, 2009-12)
Ag receptor allelic exclusion is thought to occur through monoallelic initiation and subsequent feedback inhibition of recombinational accessibility. However, our previous analysis of mice containing a V(D)J recombination ...
Parkinson's Disease Patient-Derived Induced Pluripotent Stem Cells Free of Viral Reprogramming Factors
(Elsevier B.V., 2009-03)
Induced pluripotent stem cells (iPSCs) derived from somatic cells of patients represent a powerful tool for biomedical research and may provide a source for replacement therapies. However, the use of viruses encoding the ...
Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis
(Oxford University Press, 2005-09)
We describe a large-scale random approach termed reduced representation bisulfite sequencing (RRBS) for analyzing and comparing genomic methylation patterns. BglII restriction fragments were size-selected to 500–600 bp, ...