Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator

Author(s)
Shemesh, Or A.; Linghu, Changyang; Piatkevich, Kiryl D.; Goodwin, Daniel; Celiker, Orhan Tunc; ... Show more
Thumbnail
DownloadAccepted version (3.321Mb)
Publisher with Creative Commons License
Terms of use
Creative Commons Attribution-NonCommercial-NoDerivs License http://creativecommons.org/licenses/by-nc-nd/4.0/
Metadata
Show full item record
Abstract
© 2020 Elsevier Inc. One-photon fluorescent imaging of calcium signals can capture the activity of hundreds of neurons across large fields of view but suffers from crosstalk from neuropil. Shemesh et al. engineer cell-body-targeted variants of fluorescent calcium indicators and show in mice and zebrafish that artifactual spikes and correlations are greatly reduced.
Date issued
2020-08
URI
https://hdl.handle.net/1721.1/133248.2
Department
Massachusetts Institute of Technology. Media Laboratory; Massachusetts Institute of Technology. Department of Biological Engineering; Massachusetts Institute of Technology. Center for Neurobiological Engineering; Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences; McGovern Institute for Brain Research at MIT; Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science; Picower Institute for Learning and Memory; Massachusetts Institute of Technology. Department of Mechanical Engineering; Massachusetts Institute of Technology. Department of Biology; Koch Institute for Integrative Cancer Research at MIT
Journal
Neuron
Publisher
Elsevier BV
ISSN
0896-6273
Collections

Version History

VersionItemDateSummary
21721.1/133248.2*2022-06-13T15:09:13ZPublication information verified/added.
11721.1/1332482021-10-27T19:51:46Z

*Selected version